Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146278
Title: Duplex formation in a G-quadruplex bulge
Authors: Ngoc Nguyen, Thi Quynh
Lim, Kah Wai
Phan, Anh Tuân
Keywords: Science::Chemistry
Issue Date: 2020
Source: Ngoc Nguyen, T. Q., Lim, K. W., & Phan, A. T. (2020). Duplex formation in a G-quadruplex bulge. Nucleic Acids Research, 48(18), 10567-10575. doi:10.1093/nar/gkaa738
Project: NRF-NRFI2017-09
Journal: Nucleic Acids Research
Abstract: Beyond the consensus definition of G-quadruplex-forming motifs with tracts of continuous guanines, G-quadruplexes harboring bulges in the G-tetrad core are prevalent in the human genome. Here, we study the incorporation of a duplex hairpin within a bulge of a G-quadruplex. The NMR solution structure of a G-quadruplex containing a duplex bulge was resolved, revealing the structural details of the junction between the duplex bulge and the G-quadruplex. Unexpectedly, instead of an orthogonal connection the duplex stem was observed to stack below the G-quadruplex forming a unique quadruplex-duplex junction. Breaking up of the immediate base pair step at the junction, coupled with a narrowing of the duplex groove within the context of the bulge, led to a progressive transition between the quadruplex and duplex segments. This study revealed that a duplex bulge can be formed at various positions of a G-quadruplex scaffold. In contrast to a non-structured bulge, the stability of a G-quadruplex slightly increases with an increase in the duplex bulge size. A G-quadruplex structure containing a duplex bulge of up to 33 nt in size was shown to form, which was much larger than the previously reported 7-nt bulge. With G-quadruplexes containing duplex bulges representing new structural motifs with potential biological significance, our findings would broaden the definition of potential G-quadruplex-forming sequences.
URI: https://hdl.handle.net/10356/146278
ISSN: 0305-1048
DOI: 10.1093/nar/gkaa738
Rights: © 2020 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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