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https://hdl.handle.net/10356/146336
Title: | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium | Authors: | Poenar, Daniel Puiu Yang, Guang Wan, Wei Keat Feng, Shilun |
Keywords: | Engineering::Electrical and electronic engineering | Issue Date: | 2020 | Source: | Poenar, D. P., Yang, G., Wan, W. K., & Feng, S. (2020). Low-Cost Method and Biochip for Measuring the Trans-Epithelial Electrical Resistance (TEER) of Esophageal Epithelium. Materials, 13(10), 2354-. doi:10.3390/ma13102354 | Project: | 2017-T1-002-080 RG 151/17 |
Journal: | Materials | Abstract: | Trans-epithelial electrical resistance (TEER) is a good indicator of the barrier integrity of epithelial tissues and is often employed in biomedical research as an effective tool to assess ion transport and permeability of tight junctions. The Ussing chamber is the gold standard for measuring TEER of tissue specimens, but it has major drawbacks: it is a macroscopic method that requires a careful and labor intensive sample mounting protocol, allows a very limited viability for the mounted sample, has large parasitic components and low throughput as it cannot perform multiple simultaneous measurements, and this sophisticated and delicate apparatus has a relatively high cost. This paper demonstrates a low-cost home-made "sandwich ring" method which was used to measure the TEER of tissue specimens effectively. This method inspired the subsequent design of a biochip fabricated using standard soft lithography and laser engraving technologies, with which the TEER of pig epithelial tissues was measured. Moreover, it was possible to temporarily preserve the tissue specimens for days in the biochip and monitor the TEER continuously. Tissue responses after exposure tests to media of various pH values were also successfully recorded using the biochip. All these demonstrate that this biochip could be an effective, cheaper, and easier to use Ussing chamber substitute that may have relevant applications in clinical practice. | URI: | https://hdl.handle.net/10356/146336 | ISSN: | 1996-1944 | DOI: | 10.3390/ma13102354 | Schools: | School of Electrical and Electronic Engineering | Research Centres: | Centre for Bio Devices and Signal Analysis (VALENS) | Rights: | © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/) | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | EEE Journal Articles |
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