Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146420
Title: A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation
Authors: Ong, Hon Shing
Tey, Kai Yuan
Ke, Mengyuan
Tan, Bingyao
Chua, Jacqueline
Schmetterer, Leopold
Mehta, Jodhbir Singh
Ang, Marcus
Keywords: Engineering::Chemical engineering
Issue Date: 2021
Source: Ong, H. S., Tey, K. Y., Ke, M., Tan, B., Chua, J., Schmetterer, L., . . . Ang, M. (2021). A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation. Scientific Reports, 11(1), 1212-. doi:10.1038/s41598-020-80099-2
Project: CG/C010A/2017
OFIRG/0048/2017
OFLCG/004c/2018
Journal: Scientific Reports
Abstract: The current assessment of corneal vascularisation (CV) relies on slit-lamp examination, which may be subjective. Dye-based angiographies, like indocyanine green angiography (ICGA), allows for good visualisation of anterior segment blood vessels. However, ICGA is invasive and can be associated with systemic adverse effects. Anterior segment optical coherence tomography angiography (AS-OCTA) is a non-invasive tool that has been shown to successfully delineate CV. However, there are no previous studies that have reported if AS-OCTA can determine CV stage and activity. We used an established CV model in rabbits to examine serial AS-OCTA scans of CV development and regression following treatment with anti-vascular endothelial growth factor. We compared AS-OCTA derived vascular measurements to that of ICGA determined vessel leakage and CV staging. Our results showed that AS-OCTA vessel densities and vessel branch area significantly correlated with the severity of CV based on ICGA (all p ≤ 0.05). We also found that AS-OCTA vessel densities correlated with ICGA vessel leakage time, following an inverse linear relationship (r2 = - 0.726, p < 0.01). Changes in aqueous levels of CXCL-12 and PIGF cytokines significantly correlated with AS-OCTA vessel densities (r2 = 0.736 and r2 = 0.731 respectively, all p < 0.05). In summary, we found that AS-OCTA derived vessel parameters may be useful for assessing CV severity, while vessel density correlates with CV activity and leakage. Thus, our pilot animal model study suggests that AS-OCTA may be a useful non-invasive imaging tool to provide objective assessment of CV to examine progression or response in treatment, which requires confirmation in clinical studies.
URI: https://hdl.handle.net/10356/146420
ISSN: 2045-2322
DOI: 10.1038/s41598-020-80099-2
Schools: School of Chemical and Biomedical Engineering 
School of Materials Science and Engineering 
Rights: © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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