Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146421
Title: Modulation of human mesenchymal stem cells by electrical stimulation using an enzymatic biofuel cell
Authors: Jeon, Won-Yong
Mun, Seyoung
Ng, Wei Beng
Kang, Keunsoo
Han, Kyudong
Hwang, Sohyun
Kim, Hyug-Han
Lee, Jae Ho
Keywords: Engineering::Materials
Issue Date: 2021
Source: Jeon, W.-Y., Mun, S., Ng, W. B., Kang, K., Han, K., Hwang, S., . . . Lee, J. H. (2021). Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell. Catalysts, 11(1), 62-. doi:10.3390/catal11010062
Journal: Catalysts
Abstract: Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up-or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.
URI: https://hdl.handle.net/10356/146421
ISSN: 2073-4344
DOI: 10.3390/catal11010062
Rights: © 2021 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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