Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146529
Title: High resolution, label-free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell
Authors: Sharma, Arunima
Ishak, Noreen
Teoh, Swee-Hin
Pramanik, Manojit
Keywords: Engineering::Bioengineering
Issue Date: 2020
Source: Sharma, A., Ishak, N., Teoh, S.-H., & Pramanik, M. (2020). High resolution, label-free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell. Journal of Biophotonics, 13(4), e201960108-. doi:10.1002/jbio.201960108
Journal: Journal of Biophotonics
Abstract: Chicken embryos have been proven to be an attractive vertebrate model for biomedical research. They have helped in making significant contributions for advancements in various fields like developmental biology, cancer research and cardiovascular studies. However, a non-invasive, label-free method of imaging live chicken embryo at high resolution still needs to be developed and optimized. In this work, we have shown the potential of photoacoustic tomography (PAT) for imaging live chicken embryos cultured in bioengineered eggshells. Laser pulses at wavelengths of 532 and 740 nm were used for attaining cross-sectional images of chicken embryos at different developmental stages. Cross-sections along different depths were imaged to gain knowledge of the relative depth of different vessels and organs. Due to high optical absorption of vasculature and embryonic eye, images with good optical contrast could be acquired using this method. We have thus reported a label-free method of performing cross-sectional imaging of chicken embryos at high resolution demonstrating the capacity of PAT as a promising tool for avian embryo imaging.
URI: https://hdl.handle.net/10356/146529
ISSN: 1864-0648
DOI: 10.1002/jbio.201960108
Rights: This is the peer reviewed version of the following article: Sharma, A., Ishak, N., Teoh, S.-H., & Pramanik, M. (2020). High resolution, label-free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell. Journal of Biophotonics, 13(4), e201960108-. doi:10.1002/jbio.201960108, which has been published in final form at https://doi.org/10.1002/jbio.201960108. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: embargo_20210430
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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