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Title: Photoacoustic imaging of live chicken embryo at multiple developmental stages
Authors: Sharma, Arunima
Ishak, Noreen
Teoh, Swee-Hin
Cheung, Christine
Pramanik, Manojit
Keywords: Engineering::Bioengineering
Issue Date: 2020
Source: Sharma, A., Ishak, N., Teoh, S.-H., Cheung, C., & Pramanik, M. (2020). Photoacoustic imaging of live chicken embryo at multiple developmental stages. Proceedings of SPIE - Photons Plus Ultrasound: Imaging and Sensing 2020, 11240, 108-. doi:10.1117/12.2544534
Abstract: Chicken embryo remains a commonly used model for biomedical research due to its ease of availability, accessibility for surgical manipulations, and biological similarities with humans. Although, it is being extensively used for cancer, cardiovascular, and developmental studies, non-destructive imaging of live embryo vasculature with high optical contrast remains a challenge. In this work, photoacoustic tomography of chicken embryos developing in bioengineered eggshell was performed. Chicken embryos at different developmental stages were irradiated with laser pulses at wavelengths 532 nm and 740 nm to acquire cross sectional images at various depths. We have shown a label free method for imaging of live chicken embryo while maintaining its integrity. We demonstrated that our method has the potential to be a powerful non-invasive imaging method for studying vasculature growth and development in chicken embryos.
ISBN: 9781510632431
DOI: 10.1117/12.2544534
Rights: Copyright 2020 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Conference Papers

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