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|Title:||Genomic analysis of antimicrobial resistance and resistance plasmids in Salmonella serovars from poultry in Nigeria||Authors:||Jibril, Abdurrahman Hassan
Okeke, Iruka N.
Menéndez, Vanesa García
Olsen, John Elmerdahl
|Keywords:||Engineering::Bioengineering||Issue Date:||2021||Source:||Jibril, A. H., Okeke, I. N., Dalsgaard, A., Menéndez, V. G., & Olsen, J. E. (2021). Genomic analysis of antimicrobial resistance and resistance plasmids in Salmonella serovars from poultry in Nigeria. Antibiotics, 10(2), 99-. doi:10.3390/antibiotics10020099||Journal:||Antibiotics||Abstract:||Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventy-four isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential.||URI:||https://hdl.handle.net/10356/146671||ISSN:||2079-6382||DOI:||10.3390/antibiotics10020099||Rights:||© 2021 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SCBE Journal Articles|
Updated on May 17, 2022
Updated on May 17, 2022
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