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Title: Genomic analysis of antimicrobial resistance and resistance plasmids in Salmonella serovars from poultry in Nigeria
Authors: Jibril, Abdurrahman Hassan
Okeke, Iruka N.
Dalsgaard, Anders
Menéndez, Vanesa García
Olsen, John Elmerdahl
Keywords: Engineering::Bioengineering
Issue Date: 2021
Source: Jibril, A. H., Okeke, I. N., Dalsgaard, A., Menéndez, V. G., & Olsen, J. E. (2021). Genomic analysis of antimicrobial resistance and resistance plasmids in Salmonella serovars from poultry in Nigeria. Antibiotics, 10(2), 99-. doi:10.3390/antibiotics10020099
Journal: Antibiotics
Abstract: Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventy-four isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential.
ISSN: 2079-6382
DOI: 10.3390/antibiotics10020099
Rights: © 2021 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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