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|Title:||Reconstitution of actin polymerization via artificial cells||Authors:||Susanti||Keywords:||Science::Biological sciences::Molecular biology||Issue Date:||2021||Publisher:||Nanyang Technological University||Source:||Susanti. (2021). Reconstitution of actin polymerization via artificial cells. Doctoral thesis, Nanyang Technological University, Singapore.||Abstract:||Unilamellar liposomes are sphere-shaped vesicles which are artificially created from natural phospholipids and/or cholesterol to mimic the composition of bilayer plasma membrane. Giant Unilamellar Vesicles (GUVs) are enclosed compartments or “microreactors” which offers a way to study the effect of confinement on cellular systems. Here we demonstrated several preparation approaches of GUVs, namely water-in-oil droplets with microfluidics apparatus, one-pot assembly water-in-oil droplets, GUVs with gentle hydration method and GUVs with reverse emulsion method. Specifically, we highlighted the advantages and challenges of each approach in reconstituting actin cytoskeleton, in an attempt to mimic the interaction between actin and actin-binding proteins – AtFH1, AtVLN4, XopR, Sac6 – in the cell-like vesicles. Moreover, we had also showed that we could vary the lipid compositions to create artificial cell of desired properties, as well as manipulating the proteins-lipids interaction on the membrane interface. Overall, we established capabilities of reconstituting actin polymerization inside an artificial cell-like confinement.||URI:||https://hdl.handle.net/10356/146725||DOI:||10.32657/10356/146725||Rights:||This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).||Fulltext Permission:||embargo_20250310||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Theses|
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|SUSANTI MSc. Thesis G1902499G||1.77 MB||Adobe PDF||Under embargo until Mar 10, 2025|
Updated on Jul 28, 2021
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