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|Title:||Hydroxyapatite particles induced modulation of collagen expression and secretion in primary human dermal fibroblasts||Authors:||Rakshit, Moumita
Toh, Li Zhen
Lee, Ying Shi
Lai, Hui Ying
Wong, Tina T.
Ng, Kee Woei
Science::Biological sciences::Molecular biology
|Issue Date:||2020||Source:||Rakshit, M., Gautam, A., Toh, L. Z., Lee, Y. S., Lai, H. Y., Wong, T. T., & Ng, K. W. (2020). Hydroxyapatite particles induced modulation of collagen expression and secretion in primary human dermal fibroblasts. International Journal of Nanomedicine, 15, 4943–4956. doi:10.2147/IJN.S245500||Project:||SHS-NTU/028/2016||Journal:||International Journal of Nanomedicine||Abstract:||Background Hydroxyapatite (HA) [Ca5(PO4)3(OH)] is a naturally occurring calcium phosphate which makes up 60–70% of the dry weight of human bones. Nano-scale HA particles are increasingly being used as carriers for controlled and targeted delivery of bioactive agents like drugs, proteins, and nucleic acids due to their high porosity, negative charge, and biodegradability. Purpose Although much effort has been devoted to understanding the delivery kinetics and effects of the payloads in such carriers, a thorough understanding of the influence of the carriers themselves is lacking. Methods HA particles (300 µg/mL) were administered to primary human dermal fibroblasts (HDFs). The uptake and intracellular localization of the particles were determined by flow cytometry, confocal imaging, and transmission electron microscopy (TEM). Immunological assays and PCR were performed to determine the levels of pro-inflammatory cytokines and collagens in cell lysates and media supernatant. Results The current study explores the effects of poly-dispersed HA particles on primary HDFs as a model system. The majority of the particles were determined to range between 150 and 200 nm in diameter. Upon exposure to HA suspensions, primary HDFs internalized the particles by endocytosis within 6 hours of exposure, showing maximum uptake at 72 hours following which the particles were exocytosed by 168 hours. This correlated to reduced secretion of various pro-inflammatory and pro-collagenic cytokines. Biochemical analysis further revealed a reduction in Type I collagen expression and secretion.||URI:||https://hdl.handle.net/10356/146774||ISSN:||1176-9114||DOI:||10.2147/IJN.S245500||Rights:||© 2020 Rakshit et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
Updated on May 27, 2022
Updated on May 27, 2022
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