Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/146958
Title: Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis
Authors: Shen, Yuping
Wang, Man
Chen, Yufei
Xu, Lili
Lu, Yi
Zhou, Yiying
Tam, James P.
Han, Feng
Yang, Huan
Jia, Xiaobin
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Shen, Y., Wang, M., Chen, Y., Xu, L., Lu, Y., Zhou, Y., Tam, J. P., Han, F., Yang, H. & Jia, X. (2019). Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis. Enzyme and Microbial Technology, 121, 51-58. https://dx.doi.org/10.1016/j.enzmictec.2018.12.002
Journal: Enzyme and Microbial Technology
Abstract: Sagittatoside B, a rare secondary flavonol glycoside in Epimedii Folium, has much better in vivo bioactivities than its original glycoside epimedin B. Its preparation methods, such as acidic hydrolysis, are of low efficiency, and byproducts are generated. The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. β-glucanase was selected from five commercial enzymes based on the best catalysis performance. After optimization of the conditions, the biphase system was constructed with propyl acetate and HAc-NaAc buffer (pH 4.5) (1:1, v/v) containing β-glucanase/epimedin B (1:2, w/w), and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin B was completely hydrolyzed to sagittatoside B, and 95.7% of the product was transferred into the organic phase. Moreover, a high conversion ratio of 94.0% was achieved, even after the enzyme solution was used for six cycles. Additionally, the procedure was much simplified compared with conventional enzymatic hydrolysis. The newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside B in industrial applications.
URI: https://hdl.handle.net/10356/146958
ISSN: 0141-0229
DOI: 10.1016/j.enzmictec.2018.12.002
Schools: School of Biological Sciences 
Rights: © 2018 Elsevier Inc. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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