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dc.contributor.authorShen, Yupingen_US
dc.contributor.authorWang, Manen_US
dc.contributor.authorChen, Yufeien_US
dc.contributor.authorXu, Lilien_US
dc.contributor.authorLu, Yien_US
dc.contributor.authorZhou, Yiyingen_US
dc.contributor.authorTam, James P.en_US
dc.contributor.authorHan, Fengen_US
dc.contributor.authorYang, Huanen_US
dc.contributor.authorJia, Xiaobinen_US
dc.identifier.citationShen, Y., Wang, M., Chen, Y., Xu, L., Lu, Y., Zhou, Y., Tam, J. P., Han, F., Yang, H. & Jia, X. (2019). Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis. Enzyme and Microbial Technology, 121, 51-58.
dc.description.abstractSagittatoside B, a rare secondary flavonol glycoside in Epimedii Folium, has much better in vivo bioactivities than its original glycoside epimedin B. Its preparation methods, such as acidic hydrolysis, are of low efficiency, and byproducts are generated. The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. β-glucanase was selected from five commercial enzymes based on the best catalysis performance. After optimization of the conditions, the biphase system was constructed with propyl acetate and HAc-NaAc buffer (pH 4.5) (1:1, v/v) containing β-glucanase/epimedin B (1:2, w/w), and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin B was completely hydrolyzed to sagittatoside B, and 95.7% of the product was transferred into the organic phase. Moreover, a high conversion ratio of 94.0% was achieved, even after the enzyme solution was used for six cycles. Additionally, the procedure was much simplified compared with conventional enzymatic hydrolysis. The newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside B in industrial applications.en_US
dc.relation.ispartofEnzyme and Microbial Technologyen_US
dc.rights© 2018 Elsevier Inc. All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleConvenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysisen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.subject.keywordsEpimedin Ben_US
dc.subject.keywordsSagittatoside Ben_US
dc.description.acknowledgementThis study was supported by funding from the National Natural Science Foundation of China (81873196, 81303313), National Science and Technology Major Project for ‘Significant New Drugs Innovation and Development’ (2017ZX09301051), Jiangsu Postdoctoral Research Plan (1601163B), Six Talent Peaks Project in Jiangsu (YY-010), and Qing Lan Project (2016).en_US
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