Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/147058
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dc.contributor.authorSelão, Tiago Toscanoen_US
dc.contributor.authorWłodarczyk, Arturen_US
dc.contributor.authorNixon, Peter J.en_US
dc.contributor.authorNorling, Birgittaen_US
dc.date.accessioned2021-03-19T07:36:21Z-
dc.date.available2021-03-19T07:36:21Z-
dc.date.issued2019-
dc.identifier.citationSelão, T. T., Włodarczyk, A., Nixon, P. J. & Norling, B. (2019). Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources. Metabolic Engineering, 54, 255-263. https://dx.doi.org/10.1016/j.ymben.2019.04.013en_US
dc.identifier.issn1096-7176en_US
dc.identifier.urihttps://hdl.handle.net/10356/147058-
dc.description.abstractCyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater using oxygenic photosynthesis to fix carbon dioxide into biomass. Their other major nutritional requirements for efficient growth are sources of nitrogen (N) and phosphorus (P). As these organisms are more economically cultivated in outdoor open systems, there is a need to develop cost-effective approaches to prevent the growth of contaminating organisms, especially as the use of antibiotic selection markers is neither economically feasible nor ecologically desirable due to the risk of horizontal gene transfer. Here we have introduced a synthetic melamine degradation pathway into Syn7002 and evolved the resulting strain to efficiently use the nitrogen-rich xenobiotic compound melamine as the sole N source. We also show that expression of phosphite dehydrogenase in the absence of its cognate phosphite transporter permits growth of Syn7002 on phosphite and can be used as a selectable marker in Syn7002. We combined these two strategies to generate a strain that can grow on melamine and phosphite as sole N and P sources, respectively. This strain is able to resist deliberate contamination in large excess and should be a useful chassis for metabolic engineering and biotechnological applications using cyanobacteria.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relation.ispartofMetabolic Engineeringen_US
dc.rights© 2019 International Metabolic Engineering Society. Published by Elsevier Inc.This paper was published in Metabolic Engineering and is made available with permission of International Metabolic Engineering Society. Published by Elsevier Inc.en_US
dc.subjectScienceen_US
dc.titleGrowth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sourcesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.1016/j.ymben.2019.04.013-
dc.description.versionAccepted versionen_US
dc.identifier.pmid31063791-
dc.identifier.scopus2-s2.0-85065417968-
dc.identifier.volume54en_US
dc.identifier.spage255en_US
dc.identifier.epage263en_US
dc.subject.keywordsCyanobacteriaen_US
dc.subject.keywordsLaboratory Evolutionen_US
dc.description.acknowledgementThis work was supported by NTU grants M4080306 to BN and M4081714 to PJN. The authors would like to thank Daniela Moses (SCELCE, NTU) for assistance with whole genome sequencing reactions and the NTU Phenomics Centre for LC-MS/MS analysis of culture supernatants.en_US
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