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Title: Dynamic magnetic nanomixers for improved microarray assays by eliminating diffusion limitation
Authors: Xiong, Qirong
Lim, An Eng
Lim, Yun
Lam, Yee Cheong
Duan, Hongwei
Keywords: Engineering::Mechanical engineering
Issue Date: 2019
Source: Xiong, Q., Lim, A. E., Lim, Y., Lam, Y. C. & Duan, H. (2019). Dynamic magnetic nanomixers for improved microarray assays by eliminating diffusion limitation. Advanced Healthcare Materials, 8(6), 1801022--.
Project: ING000383-ENG
Journal: Advanced Healthcare Materials
Abstract: Microarrays are widely used in high-throughput analysis of DNA, protein, and small molecules. However, the majority of microarray assays need improved assay speed and sensitivity due to the slow molecular diffusion from bulk solutions to probe surfaces. Here, a new class of magnetic nanomixers in DNA and protein microarray assays is reported to eliminate the diffusion constraint through dynamic mixing. It is demonstrated that the dynamic nanomixers can improve the assay kinetics at least by a factor of 4 and 2 for DNA and protein microarray assays, respectively. By using the dynamic nanomixers, the sensitivities of detecting Escherichia coli O157:H7 DNA and prostate specific antigen increase by more than four-fold. The dynamic mixing also greatly reduces the spot-to-spot variation to below 10% across a broad concentration range, providing more accurate assay results. In comparison with existing methods, this magnetic nanomixer-based approach offers rapid turnaround, improved sensitivity, good accuracy, low cost, simple operation, and excellent compatibility with commercial microarrays.
ISSN: 2192-2640
DOI: 10.1002/adhm.201801022
Rights: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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