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Title: Improving quality in nanoparticle-induced cytotoxicity testing by a tiered inter-laboratory comparison study
Authors: Nelissen, Inge
Haase, Andrea
Anguissola, Sergio
Rocks, Louise
Jacobs, An
Willems, Hanny
Riebeling, Christian
Luch, Andreas
Piret, Jean-Pascal
Toussaint, Olivier
Trouiller, Bénédicte
Lacroix, Ghislaine
Gutleb, Arno C.
Contal, Servane
Diabaté, Silvia
Weiss, Carsten
Lozano-Fernández, Tamara
González-Fernández, África
Dusinska, Maria
Huk, Anna
Stone, Vicki
Kanase, Nilesh
Nocuń, Marek
Stępnik, Maciej
Meschini, Stefania
Ammendolia, Maria Grazia
Lewinski, Nastassja
Riediker, Michael
Venturini, Marco
Benetti, Federico
Topinka, Jan
Brzicova, Tana
Milani, Silvia
Rädler, Joachim
Salvati, Anna
Dawson, Kenneth A.
Keywords: Engineering::Nanotechnology
Issue Date: 2020
Source: Nelissen, I., Haase, A., Anguissola, S., Rocks, L., Jacobs, A., Willems, H., Riebeling, C., Luch, A., Piret, J., Toussaint, O., Trouiller, B., Lacroix, G., Gutleb, A. C., Contal, S., Diabaté, S., Weiss, C., Lozano-Fernández, T., González-Fernández, Á., Dusinska, M., ...Dawson, K. A. (2020). Improving quality in nanoparticle-induced cytotoxicity testing by a tiered inter-laboratory comparison study. Nanomaterials, 10(8).
Journal: Nanomaterials 
Abstract: The quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). Based on the outcome of tier 2 and a survey of laboratory practices, specific training was organized, and the MTS assay SOP was refined. This led to largely improved intra- and inter-laboratory reproducibility in tier 3. In addition, we confirmed that PS-COOH and PS-NH2 are suitable negative and positive control nanoparticles, respectively, to evaluate impact of nanomaterials on cell viability using the MTS assay. Overall, we have demonstrated that the tiered process followed here, with the use of SOPs and representative control nanomaterials, is necessary and makes it possible to achieve good inter-laboratory reproducibility, and therefore high-quality nanotoxicological data.
ISSN: 2079-4991
DOI: 10.3390/nano10081430
Schools: School of Materials Science and Engineering 
Rights: © 2020 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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