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Title: Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Authors: Goh, Falicia
Zhang, Mingzi M.
Lim, Tian Ru
Low, Kia Ngee
Nge, Choy Eng
Heng, Elena
Yeo, Wan Lin
Sirota, Fernanda L.
Crasta, Sharon
Tan, Zann
Ng, Veronica
Leong, Chung Yan
Zhang, Huibin
Lezhava, Alexander
Chen, Swaine L.
Hoon, Shawn S.
Eisenhaber, Frank
Eisenhaber, Birgit
Kanagasundaram, Yoganathan
Wong, Fong T.
Ng, Siew Bee
Keywords: Engineering::Bioengineering
Issue Date: 2020
Source: Goh, F., Zhang, M. M., Lim, T. R., Low, K. N., Nge, C. E., Heng, E., Yeo, W. L., Sirota, F. L., Crasta, S., Tan, Z., Ng, V., Leong, C. Y., Zhang, H., Lezhava, A., Chen, S. L., Hoon, S. S., Eisenhaber, F., Eisenhaber, B., Kanagasundaram, Y., ...Ng, S. B. (2020). Identification and engineering of 32 membered antifungal macrolactone notonesomycins. Microbial Cell Factories, 19(1).
Project: NRF2013-THE001-094
Journal: Microbial Cell Factories
Abstract: Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR-Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.
ISSN: 1475-2859
DOI: 10.1186/s12934-020-01328-x
Rights: © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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