Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/147976
Title: A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
Authors: Kucharski, Michal
Tripathi, Jaishree
Nayak, Sourav
Zhu, Lei
Wirjanata, Grennady
van der Pluijm, Rob W.
Dhorda, Mehul
Dondorp, Arjen
Bozdech, Zbynek
Keywords: Science::Medicine
Issue Date: 2020
Source: Kucharski, M., Tripathi, J., Nayak, S., Zhu, L., Wirjanata, G., van der Pluijm, R. W., Dhorda, M., Dondorp, A. & Bozdech, Z. (2020). A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples. Malaria Journal, 19(1). https://dx.doi.org/10.1186/s12936-020-03436-w
Project: NMRC/OFIRG/0040/2017
MOE2017-T2-2-030 (S)
Journal: Malaria Journal
Abstract: Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full-length mRNA transcripts from Plasmodium-infected blood samples is presented that can be fully streamlined for high-throughput studies.
URI: https://hdl.handle.net/10356/147976
ISSN: 1475-2875
DOI: 10.1186/s12936-020-03436-w
Rights: © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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