Please use this identifier to cite or link to this item:
|Title:||Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum||Authors:||Huang, Jiayi
Wong, Ka Ho
Tan, Wei Liang
Tay, Stephanie Victoria
Tam, James P.
|Keywords:||Science||Issue Date:||2021||Source:||Huang, J., Wong, K. H., Tan, W. L., Tay, S. V., Wang, S. & Tam, J. P. (2021). Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum. Food Chemistry, 351, 129338-. https://dx.doi.org/10.1016/j.foodchem.2021.129338||Project:||MOE2016-T3-1-003
Synzymes and Natural Products
|Journal:||Food Chemistry||Abstract:||Hyperstable cysteine-rich peptides (CRPs) represent an underexplored superfamily of bioactives in functional foods. An example is wolfberry of the Lycium barbarum family. Previously, we discovered a CRP, designated α-lybatide, from L. barbarum bark. Herein, we report the discovery of β-lybatide, a novel carboxypeptidase inhibitor belonging to a different CRP family from the wolfberry plant. Proteomic and transcriptomic analyses showed that β-lybatide contains 36 amino acids with six cysteine residues. NMR spectroscopy revealed that β-lybatide displays a knottin-like structure that renders it highly resistant to thermal, chemical and enzymatic degradation, conditions important for keeping its structural integrity in gastrointestinal tract. Biochemical assays showed that β-lybatide is a potent carboxypeptidase inhibitor which could contribute to the wolfberry biological activities. Bioinformatics analysis revealed an additional 49 β-lybatide-like plant carboxypeptidase inhibitors. Together, our results show that β-lybatide is the first and the smallest plant-derived hyperstable carboxypeptidase inhibitor discovered from a functional food.||URI:||https://hdl.handle.net/10356/148027||ISSN:||0308-8146||DOI:||10.1016/j.foodchem.2021.129338||Rights:||© 2021 Elsevier Ltd. All rights reserved. This paper was published in Food Chemistry and is made available with permission of Elsevier Ltd.||Fulltext Permission:||embargo_20220730||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
Files in This Item:
|DR NTU Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum.pdf|
|1.47 MB||Adobe PDF||Under embargo until Jul 30, 2022|
Updated on Sep 21, 2021
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.