Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/148027
Title: Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum
Authors: Huang, Jiayi
Wong, Ka Ho
Tan, Wei Liang
Tay, Stephanie Victoria
Wang, Shujing
Tam, James P.
Keywords: Science
Issue Date: 2021
Source: Huang, J., Wong, K. H., Tan, W. L., Tay, S. V., Wang, S. & Tam, J. P. (2021). Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum. Food Chemistry, 351, 129338-. https://dx.doi.org/10.1016/j.foodchem.2021.129338
Project: MOE2016-T3-1-003
Synzymes and Natural Products
Journal: Food Chemistry
Abstract: Hyperstable cysteine-rich peptides (CRPs) represent an underexplored superfamily of bioactives in functional foods. An example is wolfberry of the Lycium barbarum family. Previously, we discovered a CRP, designated α-lybatide, from L. barbarum bark. Herein, we report the discovery of β-lybatide, a novel carboxypeptidase inhibitor belonging to a different CRP family from the wolfberry plant. Proteomic and transcriptomic analyses showed that β-lybatide contains 36 amino acids with six cysteine residues. NMR spectroscopy revealed that β-lybatide displays a knottin-like structure that renders it highly resistant to thermal, chemical and enzymatic degradation, conditions important for keeping its structural integrity in gastrointestinal tract. Biochemical assays showed that β-lybatide is a potent carboxypeptidase inhibitor which could contribute to the wolfberry biological activities. Bioinformatics analysis revealed an additional 49 β-lybatide-like plant carboxypeptidase inhibitors. Together, our results show that β-lybatide is the first and the smallest plant-derived hyperstable carboxypeptidase inhibitor discovered from a functional food.
URI: https://hdl.handle.net/10356/148027
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2021.129338
Rights: © 2021 Elsevier Ltd. All rights reserved. This paper was published in Food Chemistry and is made available with permission of Elsevier Ltd.
Fulltext Permission: embargo_20220730
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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