Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/148594
Title: Multi-compartment 3D-cultured organ-on-a-chip : towards a biomimetic Lymph Node for drug development
Authors: Shanti, Aya
Samara, Bisan
Amal Abdullah
Hallfors, Nicholas
Accoto, Dino
Sapudom, Jiranuwat
Alatoom, Aseel
Teo, Jeremy
Danti, Serena
Stefanini, Cesare
Keywords: Engineering::Mechanical engineering
Issue Date: 2020
Source: Shanti, A., Samara, B., Amal Abdullah, Hallfors, N., Accoto, D., Sapudom, J., Alatoom, A., Teo, J., Danti, S. & Stefanini, C. (2020). Multi-compartment 3D-cultured organ-on-a-chip : towards a biomimetic Lymph Node for drug development. Pharmaceutics, 12(5). https://dx.doi.org/10.3390/pharmaceutics12050464
Journal: Pharmaceutics
Abstract: The interaction of immune cells with drugs and/or with other cell types should be mechanistically investigated in order to reduce attrition of new drug development. However, they are currently only limited technologies that address this need. In our work, we developed initial but significant building blocks that enable such immune-drug studies. We developed a novel microfluidic platform replicating the Lymph Node (LN) microenvironment called LN-on-a-chip, starting from design all the way to microfabrication, characterization and validation in terms of architectural features, fluidics, cytocompatibility, and usability. To prove the biomimetics of this microenvironment, we inserted different immune cell types in a microfluidic device, which showed an in-vivo-like spatial distribution. We demonstrated that the developed LN-on-a-chip incorporates key features of the native human LN, namely, (i) similarity in extracellular matrix composition, morphology, porosity, stiffness, and permeability, (ii) compartmentalization of immune cells within distinct structural domains, (iii) replication of the lymphatic fluid flow pattern, (iv) viability of encapsulated cells in collagen over the typical timeframe of immunotoxicity experiments, and (v) interaction among different cell types across chamber boundaries. Further studies with this platform may assess the immune cell function as a step forward to disclose the effects of pharmaceutics to downstream immunology in more physiologically relevant microenvironments.
URI: https://hdl.handle.net/10356/148594
ISSN: 1999-4923
DOI: 10.3390/pharmaceutics12050464
Rights: © 2020 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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