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Title: Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells
Authors: Flores-Tornero, María
Proost, Sebastian
Mutwil, Marek
Scutt, Charles P.
Dresselhaus, Thomas
Sprunck, Stefanie
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Flores-Tornero, M., Proost, S., Mutwil, M., Scutt, C. P., Dresselhaus, T. & Sprunck, S. (2019). Transcriptomics of manually isolated Amborella trichopoda egg apparatus cells. Plant Reproduction, 32(1), 15-27.
Journal: Plant Reproduction
Abstract: A protocol for the isolation of egg apparatus cells from the basal angiosperm Amborella trichopoda to generate RNA-seq data for evolutionary studies of fertilization-associated genes. Sexual reproduction is particularly complex in flowering plants (angiosperms). Studies in eudicot and monocot model species have significantly contributed to our knowledge on cell fate specification of gametophytic cells and on the numerous cellular communication events necessary to deliver the two sperm cells into the embryo sac and to accomplish double fertilization. However, for a deeper understanding of the evolution of these processes, morphological, genomic and gene expression studies in extant basal angiosperms are inevitable. The basal angiosperm Amborella trichopoda is of special importance for evolutionary studies, as it is likely sister to all other living angiosperms. Here, we report about a method to isolate Amborella egg apparatus cells and on genome-wide gene expression profiles in these cells. Our transcriptomics data revealed Amborella-specific genes and genes conserved in eudicots and monocots. Gene products include secreted proteins, such as small cysteine-rich proteins previously reported to act as extracellular signaling molecules with important roles during double fertilization. The detection of transcripts encoding EGG CELL 1 (EC1) and related prolamin-like family proteins in Amborella egg cells demonstrates the potential of the generated data set to study conserved molecular mechanisms and the evolution of fertilization-related genes and their encoded proteins.
ISSN: 2194-7953
DOI: 10.1007/s00497-019-00361-0
Rights: © 2019 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativeco, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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