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Title: A vascular-liver chip for sensitive detection of nutraceutical metabolites from human pluripotent stem cell derivatives
Authors: Yu, Fang
Goh, Yeek Teck
Li, Huan
Chakrapani, Narmada Balakrishnan
Ni, Ming
Xu, Guo Lin
Hsieh, Tseng-Ming
Toh, Yi-Chin
Cheung, Christine
Iliescu, Ciprian
Yu, Hanry
Keywords: Engineering::Bioengineering
Issue Date: 2020
Source: Yu, F., Goh, Y. T., Li, H., Chakrapani, N. B., Ni, M., Xu, G. L., Hsieh, T., Toh, Y., Cheung, C., Iliescu, C. & Yu, H. (2020). A vascular-liver chip for sensitive detection of nutraceutical metabolites from human pluripotent stem cell derivatives. Biomicrofluidics, 14(3), 034108-.
Journal: Biomicrofluidics
Abstract: Human pluripotent stem cell (hPSC) is a great resource for generating cell derivatives for drug efficiency testing. Metabolites of nutraceuticals can exert anti-inflammatory effects on blood vessels. However, the concentration of nutraceutical metabolites produced in hPSC-derived hepatocytes (hPSC-HEPs) is usually low. To enable the detection of these metabolites under the in vitro environment, we have developed a co-culture model consisting of parallel co-culture chambers and a recirculating microfluidic system with minimum fluid volume, optimal cell culture environment. The model allows cells to be exposed continuously to nutraceutical metabolites. In this perfused culturing model, hPSC-derived endothelial cells and hPSC-HEPs are co-cultured without physical contact. When an anti-inflammatory nutraceutical, quercetin, was administrated to the co-culture, higher levels of quercetin metabolites were detected on-chip compared with static control. We further induced inflammation with Interleukin-1β in the co-culture model and measured interleukin 8 (IL-8) generation. The IL-8 level was suppressed more significantly by quercetin metabolites in the perfusion co-culture, as compared to static culture. This is due to enhanced metabolites production on-chip. This microfluidic co-culture model enables in vitro screening of nutraceuticals using hPSC-derived cells.
ISSN: 1932-1058
DOI: 10.1063/5.0004286
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: Institute of Molecular and Cell Biology, A*STAR
Institute of Bioengineering and Nanotechnology, A*STAR
Research Centres: Singapore Institute of Manufacturing Technology 
Rights: © 2020 Author(s). All rights reserved. This paper was published by American Institute of Physics (AIP) in Biomicrofluidics and is made available with permission of the Author(s).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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