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Title: Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria
Authors: Kong, Tian Fook
Shen, Xinhui
Yang, Chun
Ibrahim, Imran Halimi
Keywords: Engineering::Mechanical engineering
Issue Date: 2020
Source: Kong, T. F., Shen, X., Marcos, Yang, C. & Ibrahim, I. H. (2020). Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria. Biomicrofluidics, 14(5), 054105-.
Project: SMI-2015-MA-10
Journal: Biomicrofluidics
Abstract: In this work, a dielectrophoretic impedance measurement (DEPIM) lab-on-chip device for bacteria trapping and detection of Escherichia coli, Vibrio cholerae, and Enterococcus is presented. Through the integration of SU-8 negative photoresist as a microchannel and the precise alignment of the SU-8 microchannel with the on-chip gold interdigitated microelectrodes, bacteria trapping efficiencies of up to 97.4%, 97.7%, and 37.7% were achieved for E. coli, V. cholerae, and Enterococcus, respectively. The DEPIM device enables a high detection sensitivity, which requires only a total number of 69 ± 33 E. coli cells, 9 ± 2 Vibrio cholera cells, and 36 ± 13 Enterococcus cells to observe a discernible change in system impedance for detection. Nonetheless, the corrected limit of detection for Enterococcus is 95 ± 34 after taking into consideration the lower trapping efficiency. In addition, a theoretical model is developed to allow for the direct estimation of the number of bacteria through a linear relationship with the change in the reciprocal of the overall system absolute impedance.
ISSN: 1932-1058
DOI: 10.1063/5.0024826
DOI (Related Dataset): 10.21979/N9/7JPCOJ
Rights: © 2020 Author(s). All rights reserved. This paper was published by American Institute of Physics (AIP) in Applied Physics Reviews and is made available with permission of the Author(s).
Fulltext Permission: open
Fulltext Availability: With Fulltext
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