Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/148805
Title: A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice
Authors: Amouyal, Chloé
Castel, Julien
Guay, Claudiane
Lacombe, Amélie
Denom, Jessica
Migrenne-Li, Stéphanie
Rouault, Christine
Marquet, Florian
Georgiadou, Eleni
Stylianides, Theodoros
Luquet, Serge
Le Stunff, Hervé
Scharfmann, Raphael
Clément, Karine
Rutter, Guy A.
Taboureau, Olivier
Magnan, Christophe
Regazzi, Romano
Andreelli, Fabrizio
Keywords: Science::Medicine
Issue Date: 2020
Source: Amouyal, C., Castel, J., Guay, C., Lacombe, A., Denom, J., Migrenne-Li, S., Rouault, C., Marquet, F., Georgiadou, E., Stylianides, T., Luquet, S., Le Stunff, H., Scharfmann, R., Clément, K., Rutter, G. A., Taboureau, O., Magnan, C., Regazzi, R. & Andreelli, F. (2020). A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice. EBioMedicine, 58, 102895-. https://dx.doi.org/10.1016/j.ebiom.2020.102895
Journal: EBioMedicine
Abstract: Background: Bariatric surgery is an effective treatment for type 2 diabetes. Early post-surgical enhancement of insulin secretion is key for diabetes remission. The full complement of mechanisms responsible for improved pancreatic beta cell functionality after bariatric surgery is still unclear. Our aim was to identify pathways, evident in the islet transcriptome, that characterize the adaptive response to bariatric surgery independently of body weight changes. Methods: We performed entero-gastro-anastomosis (EGA) with pyloric ligature in leptin-deficient ob/ob mice as a surrogate of Roux-en-Y gastric bypass (RYGB) in humans. Multiple approaches such as determination of glucose tolerance, GLP-1 and insulin secretion, whole body insulin sensitivity, ex vivo glucose-stimulated insulin secretion (GSIS) and functional multicellular Ca2+-imaging, profiling of mRNA and of miRNA expression were utilized to identify significant biological processes involved in pancreatic islet recovery.
URI: https://hdl.handle.net/10356/148805
ISSN: 2352-3964
DOI: 10.1016/j.ebiom.2020.102895
Rights: © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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