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https://hdl.handle.net/10356/148805
Title: | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice | Authors: | Amouyal, Chloé Castel, Julien Guay, Claudiane Lacombe, Amélie Denom, Jessica Migrenne-Li, Stéphanie Rouault, Christine Marquet, Florian Georgiadou, Eleni Stylianides, Theodoros Luquet, Serge Le Stunff, Hervé Scharfmann, Raphael Clément, Karine Rutter, Guy A. Taboureau, Olivier Magnan, Christophe Regazzi, Romano Andreelli, Fabrizio |
Keywords: | Science::Medicine | Issue Date: | 2020 | Source: | Amouyal, C., Castel, J., Guay, C., Lacombe, A., Denom, J., Migrenne-Li, S., Rouault, C., Marquet, F., Georgiadou, E., Stylianides, T., Luquet, S., Le Stunff, H., Scharfmann, R., Clément, K., Rutter, G. A., Taboureau, O., Magnan, C., Regazzi, R. & Andreelli, F. (2020). A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice. EBioMedicine, 58, 102895-. https://dx.doi.org/10.1016/j.ebiom.2020.102895 | Journal: | EBioMedicine | Abstract: | Background: Bariatric surgery is an effective treatment for type 2 diabetes. Early post-surgical enhancement of insulin secretion is key for diabetes remission. The full complement of mechanisms responsible for improved pancreatic beta cell functionality after bariatric surgery is still unclear. Our aim was to identify pathways, evident in the islet transcriptome, that characterize the adaptive response to bariatric surgery independently of body weight changes. Methods: We performed entero-gastro-anastomosis (EGA) with pyloric ligature in leptin-deficient ob/ob mice as a surrogate of Roux-en-Y gastric bypass (RYGB) in humans. Multiple approaches such as determination of glucose tolerance, GLP-1 and insulin secretion, whole body insulin sensitivity, ex vivo glucose-stimulated insulin secretion (GSIS) and functional multicellular Ca2+-imaging, profiling of mRNA and of miRNA expression were utilized to identify significant biological processes involved in pancreatic islet recovery. | URI: | https://hdl.handle.net/10356/148805 | ISSN: | 2352-3964 | DOI: | 10.1016/j.ebiom.2020.102895 | Schools: | Lee Kong Chian School of Medicine (LKCMedicine) | Rights: | © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/) | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | LKCMedicine Journal Articles |
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PIIS235239642030270X.pdf | 5.5 MB | Adobe PDF | View/Open |
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