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Title: Construction of a G-quadruplex-specific DNA endonuclease
Authors: Dang, Dung Thanh
Nguyen, Le Tuan Anh
Truong, Tuom Tinh Thi
Nguyen, Hoang Duc
Phan, Anh Tuân
Keywords: Science::Biological sciences::Molecular biology
Issue Date: 2021
Source: Dang, D. T., Nguyen, L. T. A., Truong, T. T. T., Nguyen, H. D. & Phan, A. T. (2021). Construction of a G-quadruplex-specific DNA endonuclease. Chemical Communications, 57(37), 4568-4571.
Project: MOE2018-T2-2-029
Journal: Chemical Communications 
Abstract: We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.
ISSN: 1359-7345
DOI: 10.1039/d0cc05890d
Rights: © 2021 The Author(s) (Royal Society of Chemistry). All rights reserved. This paper was published in Chemical Communications and is made available with permission of The Author(s) (Royal Society of Chemistry).
Fulltext Permission: embargo_20220412
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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