Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/150186
Title: Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm
Authors: Chen, Si
Liu, Xinyu
Wang, Nanshuo
Ding, Qianshan
Wang, Xianghong
Ge, Xin
Bo, En
Yu, Xiaojun
Yu, Honggang
Xu, Chenjie
Liu, Linbo
Keywords: Engineering::Bioengineering
Issue Date: 2019
Source: Chen, S., Liu, X., Wang, N., Ding, Q., Wang, X., Ge, X., Bo, E., Yu, X., Yu, H., Xu, C. & Liu, L. (2019). Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm. Journal of Biophotonics, 12(9), e201900073-. https://dx.doi.org/10.1002/jbio.201900073
Project: NMRC/CBRG/0036/2013
NRF-CRP13-2014-05
2018-T1-001-144
H17/01/a0/008
NAM/15005
Journal: Journal of Biophotonics
Abstract: Imaging nuclei of keratinocytes in the stratified squamous epithelium has been a subject of intense research since nucleus associated cellular atypia is the key criteria for the screening and diagnosis of epithelial cancers and their precursors. However, keratinocyte nuclei have been reported to be either low scattering or high scattering, so that these inconsistent reports might have led to misinterpretations of optical images, and more importantly, hindered the establishment of optical diagnostic criteria. We disclose that they are generally low scattering in the core using Micro‐optical coherence tomography (μOCT) of 1.28‐μm axial resolution in vivo; those previously reported “high scattering” or “bright” signals from nuclei are likely from the nucleocytoplasmic boundary, and the low‐scattering nuclear cores were missed possibly due to insufficient axial resolutions (~4μm). It is further demonstrated that the high scattering signals may be associated with flattening of nuclei and cytoplasmic glycogen accumulation, which are valuable cytologic hallmarks of cell maturation.
URI: https://hdl.handle.net/10356/150186
ISSN: 1864-063X
DOI: 10.1002/jbio.201900073
Rights: This is the accepted version of the following article: Chen, S., Liu, X., Wang, N., Ding, Q., Wang, X., Ge, X., Bo, E., Yu, X., Yu, H., Xu, C. & Liu, L. (2019). Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm. Journal of Biophotonics, 12(9), which has been published in final form at https://doi.org/10.1002/jbio.201900073. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html].
Fulltext Permission: open
Fulltext Availability: With Fulltext
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