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Title: Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms
Authors: Nilsson, Martin
Jakobsen, Tim Holm
Givskov, Michael
Twetman, Svante
Tolker-Nielsen, Tim
Keywords: Engineering::Bioengineering
Issue Date: 2019
Source: Nilsson, M., Jakobsen, T. H., Givskov, M., Twetman, S. & Tolker-Nielsen, T. (2019). Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. Microbiology, 165(3), 334-342.
Journal: Microbiology 
Abstract: Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans. The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans, and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.
ISSN: 1350-0872
DOI: 10.1099/mic.0.000773
Rights: © 2019 The Authors (published by Microbiology Society). This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCELSE Journal Articles

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