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Title: Response of microbial membranes to butanol : interdigitation vs. disorder
Authors: Guo, Jingjing
Ho, James Chin Shing
Chin, Hokyun
Mark, Alan E.
Zhou, Cheng
Kjelleberg, Staffan
Liedberg, Bo
Parikh, Atul N.
Cho, Nam-Joon
Hinks, Jamie
Mu, Yuguang
Seviour, Thomas
Keywords: Engineering::Materials
Issue Date: 2019
Source: Guo, J., Ho, J. C. S., Chin, H., Mark, A. E., Zhou, C., Kjelleberg, S., Liedberg, B., Parikh, A. N., Cho, N., Hinks, J., Mu, Y. & Seviour, T. (2019). Response of microbial membranes to butanol : interdigitation vs. disorder. Physical Chemistry Chemical Physics, 21(22), 11903-11915.
Project: M4360005 
Journal: Physical Chemistry Chemical Physics 
Abstract: Biobutanol production by fermentation is potentially a sustainable alternative to butanol production from fossil fuels. However, the toxicity of butanol to fermentative bacteria, resulting largely from cell membrane fluidization, limits production titers and is a major factor limiting the uptake of the technology. Here, studies were undertaken, in vitro and in silico, on the butanol effects on a representative bacterial (i.e. Escherichia coli) inner cell membrane. A critical butanol : lipid ratio for stability of 2 : 1 was observed, computationally, consistent with complete interdigitation. However, at this ratio the bilayer was ∼20% thicker than for full interdigitation. Furthermore, butanol intercalation induced acyl chain bending and increased disorder, measured as a 27% lateral diffusivity increase experimentally in a supported lipid bilayer. There was also a monophasic Tm reduction in butanol-treated large unilamellar vesicles. Both behaviours are inconsistent with an interdigitated gel. Butanol thus causes only partial interdigitation at physiological temperatures, due to butanol accumulating at the phospholipid headgroups. Acyl tail disordering (i.e. splaying and bending) fills the subsequent voids. Finally, butanol short-circuits the bilayer and creates a coupled system where interdigitated and splayed phospholipids coexist. These findings will inform the design of strategies targeting bilayer stability for increasing biobutanol production titers.
ISSN: 1463-9076
DOI: 10.1039/c9cp01469a
Rights: © 2019 The Owner Societies. All rights reserved. This paper was published by Royal Society of Chemistry in Physical Chemistry Chemical Physics and is made available with permission of The Owner Societies.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles
SBS Journal Articles
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SCELSE Journal Articles

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