Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/150808
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dc.contributor.authorKrishnamurthy, Sangeethaen_US
dc.contributor.authorMuthukumaran, Padmalosinien_US
dc.contributor.authorJayakumar, Muthu Kumara Gnanasammandhanen_US
dc.contributor.authorLisse, Domeniken_US
dc.contributor.authorMasurkar, Nihar D.en_US
dc.contributor.authorXu, Chenjieen_US
dc.contributor.authorChan, Juliana M.en_US
dc.contributor.authorDrum, Chester L.en_US
dc.date.accessioned2021-08-01T15:04:43Z-
dc.date.available2021-08-01T15:04:43Z-
dc.date.issued2019-
dc.identifier.citationKrishnamurthy, S., Muthukumaran, P., Jayakumar, M. K. G., Lisse, D., Masurkar, N. D., Xu, C., Chan, J. M. & Drum, C. L. (2019). Surface protein engineering increases the circulation time of a cell membrane-based nanotherapeutic. Nanomedicine: Nanotechnology, Biology, and Medicine, 18, 169-178. https://dx.doi.org/10.1016/j.nano.2019.02.024en_US
dc.identifier.issn1549-9634en_US
dc.identifier.urihttps://hdl.handle.net/10356/150808-
dc.description.abstractMammalian cell membranes are often incompatible with chemical modifications typically used to increase circulation half-life. Using cellular nanoghosts as a model, we show that proline-alanine-serine (PAS) peptide sequences expressed on the membrane surface can extend the circulation time of a cell membrane derived nanotherapeutic. Membrane expression of a PAS 40 repeat sequence decreased protein binding and resulted in a 90% decrease in macrophage uptake when compared with non-PASylated controls (P ≤ 0.05). PASylation also extended circulation half-life (t1/2 = 37 h) compared with non-PASylated controls (t1/2 = 10.5 h) (P ≤ 0.005), resulting in ~7-fold higher in vivo serum concentrations at 24 h and 48 h (P ≤ 0.005). Genetically engineered membrane expression of PAS repeats may offer an alternative to PEGylation and provide extended circulation times for cellular membrane-derived nanotherapeutics.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.language.isoenen_US
dc.relationNMRC/CSAINV17nov-0008en_US
dc.relationRG 131/15en_US
dc.relation.ispartofNanomedicine: Nanotechnology, Biology, and Medicineen_US
dc.rights© 2019 Elsevier Inc. All rights reserved.en_US
dc.subjectScience::Medicineen_US
dc.titleSurface protein engineering increases the circulation time of a cell membrane-based nanotherapeuticen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.organizationTranslational Laboratory in Genetic Medicine, A*STARen_US
dc.identifier.doi10.1016/j.nano.2019.02.024-
dc.identifier.pmid30853651-
dc.identifier.scopus2-s2.0-85063883998-
dc.identifier.volume18en_US
dc.identifier.spage169en_US
dc.identifier.epage178en_US
dc.subject.keywordsPASylationen_US
dc.subject.keywordsNanoghostsen_US
dc.description.acknowledgementCLD acknowledges support from the National Medical Research Council, NMRC/CSAINV17nov-0008, XCJ thanks support by A*STAR Biomedical Research Council (IAF-PP grant), and Singapore Ministry of Education Tier-1 Academic Research Funds (RG 131/15).en_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:LKCMedicine Journal Articles

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