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https://hdl.handle.net/10356/150958
Title: | Direct isolation of circulating extracellular vesicles from blood for vascular risk profiling in type 2 diabetes mellitus | Authors: | Tay, Hui Min Leong, Sheng Yuan Xu, Xiaohan Kong, Fang Upadya, Megha Dalan, Rinkoo Tay, Chor Yong Dao, Ming Suresh, Subra Hou, Han Wei |
Keywords: | Engineering::Nanotechnology Science::Biological sciences Engineering::Bioengineering |
Issue Date: | 2021 | Source: | Tay, H. M., Leong, S. Y., Xu, X., Kong, F., Upadya, M., Dalan, R., Tay, C. Y., Dao, M., Suresh, S. & Hou, H. W. (2021). Direct isolation of circulating extracellular vesicles from blood for vascular risk profiling in type 2 diabetes mellitus. Lab On a Chip, 21(13), 2511-2523. https://dx.doi.org/10.1039/d1lc00333j | Project: | ING-000539 BIO IGN ING-001058 BIO IGN RG53/18 |
Journal: | Lab on a Chip | Abstract: | Extracellular vesicles (EVs) are key mediators of communication among cells, and clinical utilities of EVs-based biomarkers remain limited due to difficulties in isolating EVs from whole blood reliably. We report a novel inertial-based microfluidic platform for direct isolation of nanoscale EVs (exosomes, 50 to 200 nm) and medium-sized EVs (microvesicles, 200 nm to 1 μm) from blood with high efficiency (three-fold increase in EV yield compared to ultracentrifugation). In a pilot clinical study of healthy (n = 5) and type 2 diabetes mellitus (T2DM, n = 9) subjects, we detected higher EV levels in T2DM patients (P < 0.05), and identified a subset of "high-risk" T2DM subjects with abnormally high (∼10-fold to 50-fold) amounts of platelet (CD41a+) or leukocyte-derived (CD45+) EVs. Our in vitro endothelial cell assay further revealed that EVs from "high-risk" T2DM subjects induced significantly higher vascular inflammation (ICAM-1 expression) (P < 0.05) as compared to healthy and non-"high-risk" T2DM subjects, reflecting a pro-inflammatory phenotype. Overall, the EV isolation tool is scalable, and requires less manual labour, cost and processing time. This enables further development of EV-based diagnostics, whereby a combined immunological and functional phenotyping strategy can potentially be used for rapid vascular risk stratification in T2DM. | URI: | https://hdl.handle.net/10356/150958 | ISSN: | 1473-0197 | DOI: | 10.1039/d1lc00333j | Schools: | School of Biological Sciences School of Mechanical and Aerospace Engineering School of Materials Science and Engineering Lee Kong Chian School of Medicine (LKCMedicine) |
Organisations: | Tan Tock Seng Hospital | Rights: | © 2021 The Royal Society of Chemistry. All rights reserved. | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
Appears in Collections: | LKCMedicine Journal Articles MAE Journal Articles MSE Journal Articles SBS Journal Articles |
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