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Title: Direct isolation of circulating extracellular vesicles from blood for vascular risk profiling in type 2 diabetes mellitus
Authors: Tay, Hui Min
Leong, Sheng Yuan
Xu, Xiaohan
Kong, Fang
Upadya, Megha
Dalan, Rinkoo
Tay, Chor Yong
Dao, Ming
Suresh, Subra
Hou, Han Wei
Keywords: Engineering::Nanotechnology
Science::Biological sciences
Issue Date: 2021
Source: Tay, H. M., Leong, S. Y., Xu, X., Kong, F., Upadya, M., Dalan, R., Tay, C. Y., Dao, M., Suresh, S. & Hou, H. W. (2021). Direct isolation of circulating extracellular vesicles from blood for vascular risk profiling in type 2 diabetes mellitus. Lab On a Chip, 21(13), 2511-2523.
Project: ING-000539 BIO IGN
ING-001058 BIO IGN
Journal: Lab on a Chip
Abstract: Extracellular vesicles (EVs) are key mediators of communication among cells, and clinical utilities of EVs-based biomarkers remain limited due to difficulties in isolating EVs from whole blood reliably. We report a novel inertial-based microfluidic platform for direct isolation of nanoscale EVs (exosomes, 50 to 200 nm) and medium-sized EVs (microvesicles, 200 nm to 1 μm) from blood with high efficiency (three-fold increase in EV yield compared to ultracentrifugation). In a pilot clinical study of healthy (n = 5) and type 2 diabetes mellitus (T2DM, n = 9) subjects, we detected higher EV levels in T2DM patients (P < 0.05), and identified a subset of "high-risk" T2DM subjects with abnormally high (∼10-fold to 50-fold) amounts of platelet (CD41a+) or leukocyte-derived (CD45+) EVs. Our in vitro endothelial cell assay further revealed that EVs from "high-risk" T2DM subjects induced significantly higher vascular inflammation (ICAM-1 expression) (P < 0.05) as compared to healthy and non-"high-risk" T2DM subjects, reflecting a pro-inflammatory phenotype. Overall, the EV isolation tool is scalable, and requires less manual labour, cost and processing time. This enables further development of EV-based diagnostics, whereby a combined immunological and functional phenotyping strategy can potentially be used for rapid vascular risk stratification in T2DM.
ISSN: 1473-0197
DOI: 10.1039/d1lc00333j
Rights: © 2021 The Royal Society of Chemistry. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles
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