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Title: Cationic poly([R]-3-hydroxybutyrate) copolymers as antimicrobial agents
Authors: Liow, Sing Shy
Chee, Pei Lin
Owh, Cally
Zhang, Kangyi
Zhou, Yubin
Gao, Feng
Lakshminarayanan, Rajamani
Loh, Xian Jun
Keywords: Engineering::Materials
Issue Date: 2019
Source: Liow, S. S., Chee, P. L., Owh, C., Zhang, K., Zhou, Y., Gao, F., Lakshminarayanan, R. & Loh, X. J. (2019). Cationic poly([R]-3-hydroxybutyrate) copolymers as antimicrobial agents. Macromolecular Bioscience, 19(4), 1800466-.
Project: 1325400026
Journal: Macromolecular Bioscience
Abstract: Poly([R]-3-hydroxybutyrate) (PHB), a natural biodegradable polyester, has attracted much attention as a new biomaterial because of its sustainability and good biocompatibility. In this study, it is discovered that PHB can be conveniently functionalized to obtain a number of platform chain architectures that may provide a wide range of functional copolymers. In a transesterification reaction, linear (di-hydroxylated) and star shaped (tri- and tetra-hydroxylated) PHB oligomers are synthesized, followed by copolymerization with 2-(dimethylamino)ethyl methacrylate and quaternization with benzyl bromide to afford antimicrobial properties. The antimicrobial activities of the quaternary salts against clinically relevant pathogens on the interactions with outer and cytoplasmic membranes, lethal mechanisms, multipassage resistance, and synergy effect with antibiotics are investigated. Cationic PHB copolymers show effectiveness as antimicrobial agents, with minimum inhibitory concentration values 0.24-0.65 µm (or µmol dm⁻³ ) (or 32-128 µg mL⁻¹ ) against Gram-positive and Gram-negative bacteria. Modifying the copolymer architectures into star shapes results in enhanced effectiveness to disrupt the membrane integrity. Synergistic effects are attained for all the quaternized PHB derivatives when they are used together with tobramycin. Multipassage resistance does not occur in both the linear and star derivatives against Gram-negative bacteria after 20 passages.
ISSN: 1616-5187
DOI: 10.1002/mabi.201800466
Rights: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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