Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/151060
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dc.contributor.authorSaw, Nay Min Min Thawen_US
dc.contributor.authorSuwanchaikasem, Pipoben_US
dc.contributor.authorZuniga-Montanez, Rogelioen_US
dc.contributor.authorQiu, Guangleien_US
dc.contributor.authorMarzinelli, Ezequiel Miguelen_US
dc.contributor.authorWuertz, Stefanen_US
dc.contributor.authorWilliams, Rohan B. H.en_US
dc.date.accessioned2021-06-25T08:43:53Z-
dc.date.available2021-06-25T08:43:53Z-
dc.date.issued2021-
dc.identifier.citationSaw, N. M. M. T., Suwanchaikasem, P., Zuniga-Montanez, R., Qiu, G., Marzinelli, E. M., Wuertz, S. & Williams, R. B. H. (2021). Influence of extraction solvent on nontargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR). Metabolites, 11(5), 269-. https://dx.doi.org/10.3390/metabo11050269en_US
dc.identifier.issn2218-1989en_US
dc.identifier.urihttps://hdl.handle.net/10356/151060-
dc.description.abstractMetabolome profiling is becoming more commonly used in the study of complex microbial communities and microbiomes; however, to date, little information is available concerning appropriate extraction procedures. We studied the influence of different extraction solvent mixtures on untargeted metabolomics analysis of two continuous culture enrichment communities performing enhanced biological phosphate removal (EBPR), with each enrichment targeting distinct populations of polyphosphate-accumulating organisms (PAOs). We employed one non-polar solvent and up to four polar solvents for extracting metabolites from biomass. In one of the reactor microbial communities, we surveyed both intracellular and extracellular metabolites using the same set of solvents. All samples were analysed using ultra-performance liquid chromatography mass spectrometry (UPLC-MS). UPLC-MS data obtained from polar and non-polar solvents were analysed separately and evaluated using extent of repeatability, overall extraction capacity and the extent of differential abundance between physiological states. Despite both reactors demonstrating the same bioprocess phenotype, the most appropriate extraction method was biomass specific, with methanol: water (50:50 v/v) and methanol: chloroform: water (40:40:20 v/v/v) being chosen as the most appropriate for each of the two different bioreactors, respectively. Our approach provides new data on the influence of solvent choice on the untargeted surveys of the metabolome of PAO enriched EBPR communities and suggests that metabolome extraction methods need to be carefully tailored to the specific complex microbial community under study.en_US
dc.description.sponsorshipEnvironment & Water Industry Development Council (EWI)en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.language.isoenen_US
dc.relation1102-IRIS-10-02en_US
dc.relation.ispartofMetabolitesen_US
dc.rights© 2021 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectEngineering::Environmental engineeringen_US
dc.titleInfluence of extraction solvent on nontargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)en_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.contributor.researchSingapore Phenome Centreen_US
dc.identifier.doi10.3390/metabo11050269-
dc.description.versionPublished versionen_US
dc.identifier.pmid33925970-
dc.identifier.scopus2-s2.0-85105429635-
dc.identifier.issue5en_US
dc.identifier.volume11en_US
dc.identifier.spage269en_US
dc.subject.keywordsMicrobial Ecologyen_US
dc.subject.keywordsActivated Sludgeen_US
dc.description.acknowledgementThis research was supported by the Singapore National Research Foundation and the Ministry of Education under the Research Centre of Excellence Program and by a research grant from the National Research Foundation under the Environment and Water Industry Program (Project number: 1102-IRIS-10-02).en_US
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