Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/151118
Title: Scaled-up inertial microfluidics : retention system for microcarrier-based suspension cultures
Authors: Moloudi, Reza
Oh, Steve
Yang, Chun
Teo, Kim Leng
Lam, Alan Tin-Lun
Warkiani, Majid Ebrahimi
Naing, May Win
Keywords: Engineering::Bioengineering
Issue Date: 2019
Source: Moloudi, R., Oh, S., Yang, C., Teo, K. L., Lam, A. T., Warkiani, M. E. & Naing, M. W. (2019). Scaled-up inertial microfluidics : retention system for microcarrier-based suspension cultures. Biotechnology Journal, 14(5), 1800674-. https://dx.doi.org/10.1002/biot.201800674
Project: U18‐B‐017SU SIMT/18‐410006
Journal: Biotechnology Journal
Abstract: Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50 µm) mandates scaling up the channel cross-section or hydraulic diameter (DH > 0.5 mm). The Dean-coupled inertial focusing dynamics in spiral microchannels is studied broadly; however, the impacts of secondary flow on particle migration in a scaled-up spiral channel is not fully elucidated. The mechanism of particle focusing inside scaled-up rectangular and trapezoidal spiral channels (i.e., 5-10× bigger than conventional microchannels) with an aim to develop a continuous and clog-free microfiltration system for bioprocessing is studied in detail. Herein, a unique focusing based on inflection point without the aid of sheath flow is reported. This new focusing mechanism, observed in the scaled-up channels, out-performs the conventional focusing scenarios in the previously reported trapezoidal and rectangular channels. Finally, as a proof-of-concept, the utility of this device is showcased for the first time as a retention system for a cell-microcarrier (MC) suspension culture.
URI: https://hdl.handle.net/10356/151118
ISSN: 1860-6768
DOI: 10.1002/biot.201800674
Rights: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MAE Journal Articles

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