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Title: An upconversion nanoparticle enables near infrared-optogenetic manipulation of the Caenorhabditis elegans motor circuit
Authors: Ao, Yanxiao
Zeng, Kanghua
Yu, Bin
Miao, Yu
Hung, Wesley
Yu, Zhongzheng
Xue, Yanhong
Tan, Timothy Thatt Yang
Xu, Tao
Zhen, Mei
Yang, Xiangliang
Zhang, Yan
Gao, Shangbang
Keywords: Engineering::Chemical engineering
Issue Date: 2019
Source: Ao, Y., Zeng, K., Yu, B., Miao, Y., Hung, W., Yu, Z., Xue, Y., Tan, T. T. Y., Xu, T., Zhen, M., Yang, X., Zhang, Y. & Gao, S. (2019). An upconversion nanoparticle enables near infrared-optogenetic manipulation of the Caenorhabditis elegans motor circuit. ACS Nano, 13(3), 3373-3386.
Journal: ACS Nano
Abstract: Near-infrared (NIR) light penetrates tissue deeply, but its application to motor behavior stimulation has been limited by the lack of known genetic NIR light-responsive sensors. We designed and synthesized a Yb³⁺/Er³⁺/Ca²⁺-based lanthanide-doped upconversion nanoparticle (UCNP) that effectively converts 808 nm NIR light to green light emission. This UCNP is compatible with Chrimson, a cation channel activated by green light; as such, it can be used in the optogenetic manipulation of the motor behaviors of Caenorhabditis elegans. We show that this UCNP effectively activates Chrimson-expressing, inhibitory GABAergic motor neurons, leading to reduced action potential firing in the body wall muscle and resulting in locomotion inhibition. The UCNP also activates the excitatory glutamatergic DVC interneuron, leading to potentiated muscle action potential bursts and active reversal locomotion. Moreover, this UCNP exhibits negligible toxicity in neural development, growth, and reproduction, and the NIR energy required to elicit these behavioral and physiological responses does not activate the animal's temperature response. This study shows that UCNP provides a useful integrated optogenetic toolset, which may have wide applications in other experimental systems.
ISSN: 1936-0851
DOI: 10.1021/acsnano.8b09270
Rights: © 2019 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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