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Title: Metabolic engineering of Saccharomyces cerevisiae for de novo production of kaempferol
Authors: Lyu, Xiaomei
Zhao, Guili
Ng, Kuan Rei
Mark, Rita
Chen, Wei Ning
Keywords: Engineering::Chemical engineering
Issue Date: 2019
Source: Lyu, X., Zhao, G., Ng, K. R., Mark, R. & Chen, W. N. (2019). Metabolic engineering of Saccharomyces cerevisiae for de novo production of kaempferol. Journal of Agricultural and Food Chemistry, 67(19), 5596-5606.
Journal: Journal of Agricultural and Food Chemistry
Abstract: Kaempferol is a polyphenolic compound with various reported health benefits and thus harbors considerable potential for food-engineering applications. In this study, a high-yield kaempferol-producing cell factory was constructed by multiple strategies, including gene screening, elimination of the phenylethanol biosynthetic branch, optimizing the core flavonoid synthetic pathway, supplementation of precursor PEP/E4P, and mitochondrial engineering of F3H and FLS. A total of 86 mg/L of kaempferol was achieved in strain YL-4, to date the highest production titer in yeast. Furthermore, a coculture system and supplementation of surfactants were investigated, to relieve the metabolic burden as well as the low solubility/possible transport limitations of flavonoids, respectively. In the coculture system, the whole pathway was divided across two strains, resulting in 50% increased cell growth. Meanwhile, supplementation of Tween 80 in our engineered strains yielded 220 mg/L of naringenin and 200 mg/L of mixed flavonoids—among the highest production titer reported via de novo production in yeast.
ISSN: 0021-8561
DOI: 10.1021/acs.jafc.9b01329
Rights: © 2019 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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