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Title: A heparan sulfate device for the regeneration of osteochondral defects
Authors: Lee, Jonathan H.
Luo, Xiaoman
Ren, Xiafei
Tan, Tuan Chun
Smith, Raymond A. A.
Swaminathan, Kunchithapadam
Sekar, Sakthivel
Bhakoo, Kishore
Nurcombe, Victor
Hui, James H.
Cool, Simon M.
Keywords: Science::Medicine
Issue Date: 2019
Source: Lee, J. H., Luo, X., Ren, X., Tan, T. C., Smith, R. A. A., Swaminathan, K., Sekar, S., Bhakoo, K., Nurcombe, V., Hui, J. H. & Cool, S. M. (2019). A heparan sulfate device for the regeneration of osteochondral defects. Tissue Engineering, Part A, 25(5-6), 352-363.
Project: CIRG16may052
Journal: Tissue Engineering, Part A
Abstract: The regeneration of cartilage is challenging due to its low metabolic rate and avascular nature. An effective treatment for osteochondral defects remains a clinical challenge. Glycosaminoglycans such as heparan sulfate (HS) bind and enhance the activity of prochondrogenic growth factors and thus hold potential for targeted tissue regeneration without the requirement for exogenous growth factors. In this study, we examine the use of a cell- and growth factor-free HS-based technique for osteochondral repair in a rabbit model. The binding affinity between HS and several reparative proteins (TGF-β1, BMP-2, FGF-2, PDGF-BB, and VEGF₁₆₅) was studied using surface plasmon resonance. Next, an HS-impregnated gel was implanted in a large osteochondral defect in the femoral trochlea of 19 New Zealand white rabbits to study the efficacy of the treatment. Over a 12-week period, HS showed significantly enhanced subchondral bone regeneration compared with a hydrogel control. Treatment with HS also resulted in an increased presence of hyaline cartilage in the chondral region. The use of HS in osteochondral defects appears to improve both subchondral and chondral tissue repair. Our data suggest that this effect is mediated by the ability of HS to promote endogenous growth factors.
ISSN: 1937-3341
DOI: 10.1089/ten.TEA.2018.0171
Rights: © 2019 Mary Ann Liebert, Inc., publishers. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles

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