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Title: Nanomechanical microfluidic mixing and rapid labeling of silica nanoparticles using allenamide-thiol covalent linkage for bioimaging
Authors: Sreejith, Sivaramapanicker
Kishor, Rahul
Abbas, Ata
Thomas, Rijil
Yeo, Trifanny
Ranjan, Vivek Damodar
Vaidyanathan, Ramanathan
Seah, Yen Peng
Xing, Bengang
Wang, Zhenfeng
Zeng, Li
Zheng, Yuanjin
Lim, Chwee Teck
Keywords: Engineering::Electrical and electronic engineering
Issue Date: 2019
Source: Sreejith, S., Kishor, R., Abbas, A., Thomas, R., Yeo, T., Ranjan, V. D., Vaidyanathan, R., Seah, Y. P., Xing, B., Wang, Z., Zeng, L., Zheng, Y. & Lim, C. T. (2019). Nanomechanical microfluidic mixing and rapid labeling of silica nanoparticles using allenamide-thiol covalent linkage for bioimaging. ACS Applied Materials and Interfaces, 11(5), 4867-4875.
Journal: ACS Applied Materials and Interfaces 
Abstract: Rapid surface functionalization of nanomaterials using covalent linkage following "green chemistry" remains challenging, and the quest for developing simple protocols is persisting. We report a nanomechanical microfluidic approach for the coupling of allenamide functionalized organic derivatives on the surface of thiol-modified silica nanoparticles using allenamide-thiol chemistry. The coupling principle involves the use of a microfluidic surface acoustic wave device that generates acoustic streaming-based chaotic fluid micromixing that enables mixing of laterally flowing fluids containing active components. This approach was used to demonstrate the direct surface labeling of thiol-modified silica nanoparticles using a selected group of modified fluorescent tags containing allenamide handles and achieved a total labeling efficiency of 83-90%. This green approach enabled a highly efficient surface functionalization under aqueous conditions, with tunable control over the conjugation process via the applied field. The dye-labeled silica particles were characterized using various analytical techniques and found to be biocompatible with potential in live cell bioimaging. It is envisaged that this bioconjugation strategy will find numerous applications in the field of bioimaging and drug delivery.
ISSN: 1944-8244
DOI: 10.1021/acsami.8b20315
Rights: © 2019 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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