Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/151418
Title: | Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis | Authors: | Wee, Sheena Alli-Shaik, Asfa Kek, Relus Swa, Hannah L. F. Tien, Wei-Ping Lim, Vanessa W. Leo, Yee-Sin Ng, Lee-Ching Hapuarachchi, Hapuarachchige C. Gunaratne, Jayantha |
Keywords: | Science::Biological sciences | Issue Date: | 2019 | Source: | Wee, S., Alli-Shaik, A., Kek, R., Swa, H. L. F., Tien, W., Lim, V. W., Leo, Y., Ng, L., Hapuarachchi, H. C. & Gunaratne, J. (2019). Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis. Proceedings of the National Academy of Sciences of the United States of America, 116(14), 6754-6759. https://dx.doi.org/10.1073/pnas.1817867116 | Journal: | Proceedings of the National Academy of Sciences of the United States of America | Abstract: | Targeted proteomic mass spectrometry is emerging as a salient clinical diagnostic tool to track protein biomarkers. However, its strong analytical properties have not been exploited in the diagnosis and typing of flaviviruses. Here, we report the development of a sensitive and specific single-shot robust assay for flavivirus typing and diagnosis using targeted mass spectrometry technology. Our flavivirus parallel reaction monitoring assay (fvPRM) has the ability to track secreted flaviviral nonstructural protein 1 (NS1) over a broad diagnostic and typing window with high sensitivity, specificity, extendibility, and multiplexing capability. These features, pivotal and pertinent to efficient response toward flavivirus outbreaks, including newly emerging flavivirus strains, circumvent the limitations of current diagnostic assays. fvPRM thus carries high potential in positioning itself as a forerunner in delivering early and accurate diagnosis for disease management. | URI: | https://hdl.handle.net/10356/151418 | ISSN: | 0027-8424 | DOI: | 10.1073/pnas.1817867116 | Schools: | School of Biological Sciences | Rights: | © 2019 The Author(s) (Published by National Academy of Sciences). All rights reserved. | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
Appears in Collections: | SBS Journal Articles |
SCOPUSTM
Citations
20
16
Updated on Mar 25, 2024
Web of ScienceTM
Citations
20
15
Updated on Oct 28, 2023
Page view(s)
256
Updated on Mar 28, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.