Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/151418
Title: Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis
Authors: Wee, Sheena
Alli-Shaik, Asfa
Kek, Relus
Swa, Hannah L. F.
Tien, Wei-Ping
Lim, Vanessa W.
Leo, Yee-Sin
Ng, Lee-Ching
Hapuarachchi, Hapuarachchige C.
Gunaratne, Jayantha
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Wee, S., Alli-Shaik, A., Kek, R., Swa, H. L. F., Tien, W., Lim, V. W., Leo, Y., Ng, L., Hapuarachchi, H. C. & Gunaratne, J. (2019). Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis. Proceedings of the National Academy of Sciences of the United States of America, 116(14), 6754-6759. https://dx.doi.org/10.1073/pnas.1817867116
Journal: Proceedings of the National Academy of Sciences of the United States of America
Abstract: Targeted proteomic mass spectrometry is emerging as a salient clinical diagnostic tool to track protein biomarkers. However, its strong analytical properties have not been exploited in the diagnosis and typing of flaviviruses. Here, we report the development of a sensitive and specific single-shot robust assay for flavivirus typing and diagnosis using targeted mass spectrometry technology. Our flavivirus parallel reaction monitoring assay (fvPRM) has the ability to track secreted flaviviral nonstructural protein 1 (NS1) over a broad diagnostic and typing window with high sensitivity, specificity, extendibility, and multiplexing capability. These features, pivotal and pertinent to efficient response toward flavivirus outbreaks, including newly emerging flavivirus strains, circumvent the limitations of current diagnostic assays. fvPRM thus carries high potential in positioning itself as a forerunner in delivering early and accurate diagnosis for disease management.
URI: https://hdl.handle.net/10356/151418
ISSN: 0027-8424
DOI: 10.1073/pnas.1817867116
Schools: School of Biological Sciences 
Rights: © 2019 The Author(s) (Published by National Academy of Sciences). All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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