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https://hdl.handle.net/10356/151472
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tan, Sijie | en_US |
dc.contributor.author | Bajalovic, Natasa | en_US |
dc.contributor.author | Wong, Esther S. P. | en_US |
dc.contributor.author | Lin, Valerie Chun Ling | en_US |
dc.date.accessioned | 2021-06-16T07:41:42Z | - |
dc.date.available | 2021-06-16T07:41:42Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Tan, S., Bajalovic, N., Wong, E. S. P. & Lin, V. C. L. (2019). Ligand-activated progesterone receptor B activates transcription factor EB to promote autophagy in human breast cancer cells. Experimental Cell Research, 382(1), 111433-. https://dx.doi.org/10.1016/j.yexcr.2019.05.014 | en_US |
dc.identifier.issn | 0014-4827 | en_US |
dc.identifier.uri | https://hdl.handle.net/10356/151472 | - |
dc.description.abstract | Autophagy is an evolutionary conserved, self-eating process that targets cellular constituents for lysosomal degradation. Transcription factor EB (TFEB) is a master regulator of autophagy by inducing the expression of genes involved in autophagic and lysosomal degradation. In breast cancer, ligand-activated progesterone receptor has been reported to influence cancer development by manipulating the autophagy pathway. However, understanding of the mechanism that underlies this autophagic response remains limited. Herein, we report that prolonged treatment with progestin R5020 upregulates autophagy in MCF-7 human breast cancer cells via a novel interplay between progesterone receptor B (PRB) and TFEB. R5020 upregulates TFEB gene expression and protein levels in a PRB-dependent manner. Additionally, R5020 enhances the co-recruitment of PRB and TFEB to each other to facilitate TFEB nuclear localization. Once in the nucleus, TFEB induces the expression of autophagy and lysosomal genes to potentiate autophagy. Together, our findings highlight a novel functional connection between ligand-activated PRB and TFEB to modulate autophagy in MCF-7 breast cancer cells. As breast cancer development is controlled by autophagy, the progestin-PRB-TFEB transduction pathway warrants future attention as a potential therapeutic target in cancer therapy. | en_US |
dc.description.sponsorship | Ministry of Education (MOE) | en_US |
dc.language.iso | en | en_US |
dc.relation | MOE2014-T2-2-125 | en_US |
dc.relation.ispartof | Experimental Cell Research | en_US |
dc.rights | © 2019 Elsevier Inc. All rights reserved. | en_US |
dc.subject | Science::Biological sciences | en_US |
dc.title | Ligand-activated progesterone receptor B activates transcription factor EB to promote autophagy in human breast cancer cells | en_US |
dc.type | Journal Article | en |
dc.contributor.school | School of Biological Sciences | en_US |
dc.contributor.organization | Yong Loo Lin School of Medicine, National University of Singapore | en_US |
dc.identifier.doi | 10.1016/j.yexcr.2019.05.014 | - |
dc.identifier.pmid | 31100306 | - |
dc.identifier.scopus | 2-s2.0-85066095580 | - |
dc.identifier.issue | 1 | en_US |
dc.identifier.volume | 382 | en_US |
dc.identifier.spage | 111433 | en_US |
dc.subject.keywords | Autophagy | en_US |
dc.subject.keywords | Breast Cancer | en_US |
dc.description.acknowledgement | This work is supported by Singapore Ministry of Education Academic Research Fund Tier 2, MOE2014-T2-2-125. | en_US |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
Appears in Collections: | SBS Journal Articles |
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