Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/151472
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTan, Sijieen_US
dc.contributor.authorBajalovic, Natasaen_US
dc.contributor.authorWong, Esther S. P.en_US
dc.contributor.authorLin, Valerie Chun Lingen_US
dc.date.accessioned2021-06-16T07:41:42Z-
dc.date.available2021-06-16T07:41:42Z-
dc.date.issued2019-
dc.identifier.citationTan, S., Bajalovic, N., Wong, E. S. P. & Lin, V. C. L. (2019). Ligand-activated progesterone receptor B activates transcription factor EB to promote autophagy in human breast cancer cells. Experimental Cell Research, 382(1), 111433-. https://dx.doi.org/10.1016/j.yexcr.2019.05.014en_US
dc.identifier.issn0014-4827en_US
dc.identifier.urihttps://hdl.handle.net/10356/151472-
dc.description.abstractAutophagy is an evolutionary conserved, self-eating process that targets cellular constituents for lysosomal degradation. Transcription factor EB (TFEB) is a master regulator of autophagy by inducing the expression of genes involved in autophagic and lysosomal degradation. In breast cancer, ligand-activated progesterone receptor has been reported to influence cancer development by manipulating the autophagy pathway. However, understanding of the mechanism that underlies this autophagic response remains limited. Herein, we report that prolonged treatment with progestin R5020 upregulates autophagy in MCF-7 human breast cancer cells via a novel interplay between progesterone receptor B (PRB) and TFEB. R5020 upregulates TFEB gene expression and protein levels in a PRB-dependent manner. Additionally, R5020 enhances the co-recruitment of PRB and TFEB to each other to facilitate TFEB nuclear localization. Once in the nucleus, TFEB induces the expression of autophagy and lysosomal genes to potentiate autophagy. Together, our findings highlight a novel functional connection between ligand-activated PRB and TFEB to modulate autophagy in MCF-7 breast cancer cells. As breast cancer development is controlled by autophagy, the progestin-PRB-TFEB transduction pathway warrants future attention as a potential therapeutic target in cancer therapy.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.language.isoenen_US
dc.relationMOE2014-T2-2-125en_US
dc.relation.ispartofExperimental Cell Researchen_US
dc.rights© 2019 Elsevier Inc. All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleLigand-activated progesterone receptor B activates transcription factor EB to promote autophagy in human breast cancer cellsen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationYong Loo Lin School of Medicine, National University of Singaporeen_US
dc.identifier.doi10.1016/j.yexcr.2019.05.014-
dc.identifier.pmid31100306-
dc.identifier.scopus2-s2.0-85066095580-
dc.identifier.issue1en_US
dc.identifier.volume382en_US
dc.identifier.spage111433en_US
dc.subject.keywordsAutophagyen_US
dc.subject.keywordsBreast Canceren_US
dc.description.acknowledgementThis work is supported by Singapore Ministry of Education Academic Research Fund Tier 2, MOE2014-T2-2-125.en_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:SBS Journal Articles

SCOPUSTM   
Citations 20

13
Updated on Mar 24, 2024

Web of ScienceTM
Citations 20

12
Updated on Oct 28, 2023

Page view(s)

234
Updated on Mar 29, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.