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dc.contributor.authorFazil, Mobashar Hussain Urf Turabeen_US
dc.contributor.authorChalasani, Madhavi Latha Somarajuen_US
dc.contributor.authorChoong, Yeu Khaien_US
dc.contributor.authorSchmidtchen, Arturen_US
dc.contributor.authorVerma, Navin Kumaren_US
dc.contributor.authorSaravanan, Rathien_US
dc.identifier.citationFazil, M. H. U. T., Chalasani, M. L. S., Choong, Y. K., Schmidtchen, A., Verma, N. K. & Saravanan, R. (2019). A C-terminal peptide of TFPI-1 facilitates cytosolic delivery of nucleic acid cargo into mammalian cells. BBA - Biomembranes, 1862(2), 183093-.
dc.description.abstractEfficient intracellular nucleic acid delivery into mammalian cells remains a long-standing challenge owing to poor cell permeability and uptake of naked nucleic acids across the cell membrane and limited cargo stability. Conventional delivery methods have several drawbacks, such as cytotoxicity, limited cell-type applicability, low efficiency, hindrances that limit the potential of oligonucleotide delivery in functional genomics, therapeutics and diverse research applications. Thus, new approaches that are robust, safe, effective and valid across multiple cell types are much needed. Here, we demonstrate that GGL27, a TFPI-1-derived novel cationic host defence peptide, facilitates the delivery of nucleic acid cargo into the cytosol of a range of mammalian cells. The GGL27 peptide is non-cytotoxic and is internalized in a broad range of mammalian cell-types, including transformed cell lines and primary cells. GGL27 spontaneously forms complexes with nucleic acids of variable sizes, protects them from nuclease degradation, and delivers cargo effectively. Together, our observations demonstrate the versatile cell-penetrating property of GGL27, providing an excellent template for developing a simple, non-toxic peptide-based cytosolic delivery tool for wide use in biomedical research.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.description.sponsorshipSkin Research Institute of Singapore (SRIS)en_US
dc.relation.ispartofBBA - Biomembranesen_US
dc.rights© 2019 Elsevier B.V. All rights reserved.en_US
dc.titleA C-terminal peptide of TFPI-1 facilitates cytosolic delivery of nucleic acid cargo into mammalian cellsen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.organizationSkin Research Institute of Singaporeen_US
dc.subject.keywordsHost Defence Peptideen_US
dc.subject.keywordsIntracellular Gene Deliveryen_US
dc.description.acknowledgementThis work was supported by the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Postdoctoral Fellowship 2014 (L049201020), to RS. Authors acknowledge funding support, in part, by Start-Up Grants to N.K.V. (L0412290) and A.S., provided by Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, and the Skin Innovation Grant (SIG18015) to N.K.V. by the Skin Research Institute of Singapore.en_US
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