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Title: Near-infrared fluorescent macromolecular reporters for real-time imaging and urinalysis of cancer immunotherapy
Authors: He, Shasha
Li, Jingchao
Lyu, Yan
Huang, Jiaguo
Pu, Kanyi
Keywords: Engineering::Bioengineering
Issue Date: 2020
Source: He, S., Li, J., Lyu, Y., Huang, J. & Pu, K. (2020). Near-infrared fluorescent macromolecular reporters for real-time imaging and urinalysis of cancer immunotherapy. Journal of the American Chemical Society, 142(15), 7075-7082.
Project: M4081627
Journal: Journal of the American Chemical Society
Abstract: Real-time imaging of immunoactivation is imperative for cancer immunotherapy and drug discovery; however, most existing imaging agents possess "always-on" signals and thus have poor signal correlation with immune responses. Herein, renal-clearable near-infrared (NIR) fluorescent macromolecular reporters are synthesized to specifically detect an immunoactivation-related biomarker (granzyme B) for real-time evaluation of cancer immunotherapy. Composed of a peptide-caged NIR signaling moiety linked with a hydrophilic poly(ethylene glycol) (PEG) passivation chain, the reporters not only specifically activate their fluorescence by granzyme B but also passively target the tumor of living mice after systemic administration. Such granzyme B induced in vivo signals of the reporters are validated to correlate well with the populations of cytotoxic T lymphocytes (CD8⁺) and T helper (CD4⁺) cells detected in tumor tissues. By virtue of their ideal renal clearance efficiency (60% injected doses at 24 h postinjection), the reporters can be used for optical urinalysis of immunoactivation simply by detecting the status of excreted reporters. This study thus proposes a molecular optical imaging approach for noninvasive evaluation of cancer immunotherapeutic efficacy in living animals.
ISSN: 0002-7863
DOI: 10.1021/jacs.0c00659
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2020 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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