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dc.contributor.authorLuhung, Irvanen_US
dc.contributor.authorUchida, Akiraen_US
dc.contributor.authorLiLim, Serene Boon Yueanen_US
dc.contributor.authorGaultier, Nicolas Eugeneen_US
dc.contributor.authorKee, Carmonen_US
dc.contributor.authorLau, Kenny Jia Xuen_US
dc.contributor.authorGusareva, Elena S.en_US
dc.contributor.authorHeinle, Cassie Elizabethen_US
dc.contributor.authorWong, Anthonyen_US
dc.contributor.authorPremkrishnan, Balakrishnan N. V.en_US
dc.contributor.authorPurbojati, Rikky Wenangen_US
dc.contributor.authorAcerbi, Enzoen_US
dc.contributor.authorKim, Hie Limen_US
dc.contributor.authorJunqueira, Ana Carolina M.en_US
dc.contributor.authorLongford, Sharon Raeen_US
dc.contributor.authorLohar, Sachin R.en_US
dc.contributor.authorYap, Zhei Hweeen_US
dc.contributor.authorPanicker, Deepaen_US
dc.contributor.authorKoh, Yanqingen_US
dc.contributor.authorKushwaha, Kavita K.en_US
dc.contributor.authorAng, Poh Neeen_US
dc.contributor.authorPutra, Alexanderen_US
dc.contributor.authorDrautz-Moses, Daniela Isabelen_US
dc.contributor.authorSchuster, Stephan Christophen_US
dc.identifier.citationLuhung, I., Uchida, A., LiLim, S. B. Y., Gaultier, N. E., Kee, C., Lau, K. J. X., Gusareva, E. S., Heinle, C. E., Wong, A., Premkrishnan, B. N. V., Purbojati, R. W., Acerbi, E., Kim, H. L., Junqueira, A. C. M., Longford, S. R., Lohar, S. R., Yap, Z. H., Panicker, D., Koh, Y., ...Schuster, S. C. (2021). Experimental parameters defining ultra-low biomass bioaerosol analysis. Npj Biofilms and Microbiomes, 7(1), 37-.
dc.description.abstractInvestigation of the microbial ecology of terrestrial, aquatic and atmospheric ecosystems requires specific sampling and analytical technologies, owing to vastly different biomass densities typically encountered. In particular, the ultra-low biomass nature of air presents an inherent analytical challenge that is confounded by temporal fluctuations in community structure. Our ultra-low biomass pipeline advances the field of bioaerosol research by significantly reducing sampling times from days/weeks/months to minutes/hours, while maintaining the ability to perform species-level identification through direct metagenomic sequencing. The study further addresses all experimental factors contributing to analysis outcome, such as amassment, storage and extraction, as well as factors that impact on nucleic acid analysis. Quantity and quality of nucleic acid extracts from each optimisation step are evaluated using fluorometry, qPCR and sequencing. Both metagenomics and marker gene amplification-based (16S and ITS) sequencing are assessed with regard to their taxonomic resolution and inter-comparability. The pipeline is robust across a wide range of climatic settings, ranging from arctic to desert to tropical environments. Ultimately, the pipeline can be adapted to environmental settings, such as dust and surfaces, which also require ultra-low biomass analytics.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.relation.ispartofnpj Biofilms and Microbiomesen_US
dc.rights© 2021 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectScience::Biological sciencesen_US
dc.titleExperimental parameters defining ultra-low biomass bioaerosol analysisen_US
dc.typeJournal Articleen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsEnvironmental Microbiologyen_US
dc.subject.keywordsNext-generation Sequencingen_US
dc.description.acknowledgementThe work was supported by Singapore Ministry of Education Academic Research Fund Tier 3 grant (grant MOE2013-T3-1-013).en_US
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