Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/152021
Title: Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research
Authors: Low, Swee Ling
Leo, Yee Sin
Lai, Yee Ling
Lam, Sally
Tan, Hwee Huang
Wong, Judith Chui Ching
Tan, Li Kiang
Ng, Lee Ching
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Low, S. L., Leo, Y. S., Lai, Y. L., Lam, S., Tan, H. H., Wong, J. C. C., Tan, L. K. & Ng, L. C. (2021). Evaluation of eight commercial Zika virus IgM and IgG serology assays for diagnostics and research. PloS ONE, 16(1), e0244601-. https://dx.doi.org/10.1371/journal.pone.0244601
Journal: PloS ONE
Abstract: Several commercial Zika virus (ZIKV) serology assays have been developed since the recognition of ZIKV outbreaks as a Public Health Emergency of International Concern in 2016. However, test interpretation for ZIKV serology can be challenging due to antibody cross-reactivity with other flaviviruses like dengue virus (DENV). Therefore, we sought to evaluate the performance of eight commercially available ZIKV IgM and IgG assays across three testing platforms, namely, immunochromatographic tests (ICT), ELISAs and immunofluorescence tests (IIFT). The test panel comprised of 278 samples, including acute and convalescent sera or plasma from ZIKV-confirmed, DENV-confirmed, non-ZIKV and non-DENV patients, and residual sera from healthy blood donors. The ZIKV IgM and IgG serology assays yielded higher test sensitivities of 23.5% - 97.1% among ZIKV convalescent samples as compared to 5.6% - 27.8% among ZIKV acute samples; the test specificities were 63.3% - 100% among acute and convalescent DENV, non-DENV samples. Among the ELISAs and IIFTs, the Diapro ZIKV IgM ELISA demonstrated high test sensitivity (96%) and specificity (80%) when tested on early convalescent samples, while the Euroimmun ZIKV IgG ELISA yielded the highest test specificity of 97% - 100% on samples from non-ZIKV patients and healthy blood donors. For rapid ICTs, the LumiQuick IgM rapid ICT yielded low test sensitivity, suggesting its limited utility. We showed that commercial ZIKV IgM and IgG serology assays have differing test performances, with some having moderate to high test sensitivities and specificities when used in a dengue endemic setting, although there were limitations in IgG serology.
URI: https://hdl.handle.net/10356/152021
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0244601
Schools: School of Biological Sciences 
Rights: © 2021 Low et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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