Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/152078
Title: Synergistic effect of PVDF-coated PCL-TCP scaffolds and pulsed electromagnetic field on osteogenesis
Authors: Dong, Yibing
Suryani, Luvita
Zhou, Xinran
Muthukumaran, Padmalosini
Rakshit, Moumita
Yang, Fengrui
Wen, Feng
Hassanbhai, Ammar Mansoor
Parida, Kaushik
Simon, Daniel T.
Iandolo, Donata
Lee, Pooi See
Ng, Kee Woei
Teoh, Swee-Hin
Keywords: Engineering::Chemical engineering
Engineering::Materials
Issue Date: 2021
Source: Dong, Y., Suryani, L., Zhou, X., Muthukumaran, P., Rakshit, M., Yang, F., Wen, F., Hassanbhai, A. M., Parida, K., Simon, D. T., Iandolo, D., Lee, P. S., Ng, K. W. & Teoh, S. (2021). Synergistic effect of PVDF-coated PCL-TCP scaffolds and pulsed electromagnetic field on osteogenesis. International Journal of Molecular Sciences, 22(12), 6438-. https://dx.doi.org/10.3390/ijms22126438
Project: MOE2016-T2-2-108 
A18A8b0059 
Journal: International Journal of Molecular Sciences 
Abstract: Bone exhibits piezoelectric properties. Thus, electrical stimulations such as pulsed electromagnetic fields (PEMFs) and stimuli-responsive piezoelectric properties of scaffolds have been investigated separately to evaluate their efficacy in supporting osteogenesis. However, current understanding of cells responding under the combined influence of PEMF and piezoelectric properties in scaffolds is still lacking. Therefore, in this study, we fabricated piezoelectric scaffolds by functionalization of polycaprolactone-tricalcium phosphate (PCL-TCP) films with a polyvinylidene fluoride (PVDF) coating that is self-polarized by a modified breath-figure technique. The osteoinductive properties of these PVDF-coated PCL-TCP films on MC3T3-E1 cells were studied under the stimulation of PEMF. Piezoelectric and ferroelectric characterization demonstrated that scaffolds with piezoelectric coefficient d₃₃ = −1.2 pC/N were obtained at a powder dissolution temperature of 100 °C and coating relative humidity (RH) of 56%. DNA quantification showed that cell proliferation was significantly enhanced by PEMF as low as 0.6 mT and 50 Hz. Hydroxyapatite staining showed that cell mineralization was significantly enhanced by incorporation of PVDF coating. Gene expression study showed that the combination of PEMF and PVDF coating promoted late osteogenic gene expression marker most significantly. Collectively, our results suggest that the synergistic effects of PEMF and piezoelectric scaffolds on osteogenesis provide a promising alternative strategy for electrically augmented osteoinduction. The piezoelectric response of PVDF by PEMF, which could provide mechanical strain, is particularly interesting as it could deliver local mechanical stimulation to osteogenic cells using PEMF.
URI: https://hdl.handle.net/10356/152078
ISSN: 1661-6596
DOI: 10.3390/ijms22126438
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MSE Journal Articles
NEWRI Journal Articles
SCBE Journal Articles

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