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Title: Temperature-induced catch-slip to slip bond transit in Plasmodium falciparum-infected erythrocytes
Authors: Lim, Bena Ying
Thingna, Juzar
Kong, Fang
Dao, Ming
Cao, Jianshu
Lim, Chwee Teck
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Lim, B. Y., Thingna, J., Kong, F., Dao, M., Cao, J. & Lim, C. T. (2019). Temperature-induced catch-slip to slip bond transit in Plasmodium falciparum-infected erythrocytes. Biophysical Journal, 118(1), 105-116.
Journal: Biophysical Journal 
Abstract: Plasmodium falciparum malaria-infected red blood cells (IRBCs), or erythrocytes, avoid splenic clearance by adhering to host endothelium. Upregulation of endothelial receptors intercellular adhesion molecule-1 (ICAM-1) and cluster of differentiation 36 (CD36) are associated with severe disease pathology. Most in vitro studies of IRBCs interacting with these molecules were conducted at room temperature. However, as IRBCs are exposed to temperature variations between 37°C (body temperature) and 41°C (febrile temperature) in the host, it is important to understand IRBC-receptor interactions at these physiologically relevant temperatures. Here, we probe IRBC interactions against ICAM-1 and CD36 at 37 and 41°C. Single bond force-clamp spectroscopy is used to determine the bond dissociation rates and hence, unravel the nature of the IRBC-receptor interaction. The association rates are also extracted from a multiple bond flow assay using a cellular stochastic model. Surprisingly, IRBC-ICAM-1 bond transits from a catch-slip bond at 37°C toward a slip bond at 41°C. Moreover, binding affinities of both IRBC-ICAM-1 and IRBC-CD36 decrease as the temperature rises from 37 to 41°C. This study highlights the significance of examining receptor-ligand interactions at physiologically relevant temperatures and reveals biophysical insight into the temperature dependence of P. falciparum malaria cytoadherent bonds.
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.016
Rights: © 2019 Biophysical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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