Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/152343
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dc.contributor.authorTan, Xue Weien_US
dc.date.accessioned2021-08-05T02:55:42Z-
dc.date.available2021-08-05T02:55:42Z-
dc.date.issued2021-
dc.identifier.citationTan, X. W. (2021). Biological consequences of error during ribosome biogenesis. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/152343en_US
dc.identifier.urihttps://hdl.handle.net/10356/152343-
dc.description.abstractRpl24, a eukaryotic 60S ribosomal protein, forms inter-subunit bridges. Lack of Rpl24 protein may weaken inter-subunit association, introducing problems during translation such as ribosome stalling. Ribosome stalling produces stalled polypeptides which accumulate to form toxic aggregates if undegraded. Past studies usually focused on aberrant messenger RNAs (mRNAs) resulting in ribosome stalling, however, few have focused on defective ribosomes as a potential cause. Here, I aimed to show that incomplete ribosomes that may arise due to errors during ribosome biogenesis can trigger translation arrest, using Saccharomyces cerevisiae (S. cerevisiae) as a model organism. To explore impact of Rpl24 deletion on translation, I observed nascent protein aggregation and toxicity of 4 different Rpl24 constructs: 3 truncated mutants, Rpl24 with N-terminal 1-65 amino acids (aa) (Rpl24 (65)), 1-80 aa (Rpl24 (80)) and 1-111aa (Rpl24 (111)), plus the full-length protein (Rpl24 (FL)). More truncations would increasingly disrupt the inter-subunit bridge. Rpl24 (65) grows slower compared to Rpl24 (80)/((111)/(FL). Hence, Rpl24 (80) is sufficient for normal Rpl24 function, indicating that the important domain of Rpl24 lies between the 65th and 80th aa. This study provides insight into the importance of ribosomal inter-subunit bridges and mechanisms underlying ribosomal stalling.en_US
dc.language.isoenen_US
dc.publisherNanyang Technological Universityen_US
dc.subjectScience::Biological sciencesen_US
dc.titleBiological consequences of error during ribosome biogenesisen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorChoe Young Junen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.supervisoremailyjchoe@ntu.edu.sgen_US
item.grantfulltextembargo_restricted_20230802-
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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