Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153324
Full metadata record
DC FieldValueLanguage
dc.contributor.authorChng, Choon-Pengen_US
dc.contributor.authorWang, Kunen_US
dc.contributor.authorMa, Weien_US
dc.contributor.authorHsia, K. Jimmyen_US
dc.contributor.authorHuang, Changjinen_US
dc.date.accessioned2021-12-14T13:36:08Z-
dc.date.available2021-12-14T13:36:08Z-
dc.date.issued2021-
dc.identifier.citationChng, C., Wang, K., Ma, W., Hsia, K. J. & Huang, C. (2021). Chloroplast membrane lipid remodeling protects against dehydration by limiting membrane fusion and distortion. Plant Physiology, 188(1), 526-539. https://dx.doi.org/10.1093/plphys/kiab512en_US
dc.identifier.issn0032-0889en_US
dc.identifier.urihttps://hdl.handle.net/10356/153324-
dc.description.abstractDehydration damages the structural integrity of the chloroplast membrane and, consequently, the normal photosynthetic function of this organelle. Remodeling of galactolipids by converting monogalactosyl-diacylglycerol (MGDG) to digalactosyl-diacylglycerol (DGDG) and oligo-galactolipids is an effective adaptation strategy for protecting against dehydration damage to the chloroplast membrane. However, detailed molecular mechanisms are missing. In this study, by performing molecular-level simulations of bi-lamellar membranes under various dehydration conditions, we find that MGDG-to-DGDG remodeling protects the chloroplast membrane in a unique manner by simultaneously dictating both the extent and the pattern of fusion stalks formed with the apposed membrane. Specifically, MGDG-rich membranes form elongated stalks at a moderate dehydration level, whereas DGDG-rich membranes form smaller, rounded stalks. Simulations of wild-type and mutant Arabidopsis (Arabidopsis thaliana) outer chloroplast membranes further confirm that the mutant membrane without galactolipid remodeling is more susceptible to membrane fusion due to its higher MGDG content. Our work reveals the underlying physical mechanisms that govern the pattern and extent of membrane fusion structures, paving the way for rational genetic engineering of crops with improved dehydration tolerance.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relationNTU–ACE2020-07en_US
dc.relationRG92/19en_US
dc.relationM4082428en_US
dc.relationM4082352en_US
dc.relation.ispartofPlant Physiologyen_US
dc.relation.uri10.21979/N9/WTDBTDen_US
dc.rights© 2021 American Society of Plant Biologists. All rights reserved. This paper was published in Plant Physiology and is made available with permission of American Society of Plant .en_US
dc.subjectScience::Biological sciences::Biophysicsen_US
dc.titleChloroplast membrane lipid remodeling protects against dehydration by limiting membrane fusion and distortionen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.identifier.doi10.1093/plphys/kiab512-
dc.description.versionAccepted versionen_US
dc.identifier.pmid34730798-
dc.identifier.issue1en_US
dc.identifier.volume188en_US
dc.identifier.spage526en_US
dc.identifier.epage539en_US
dc.subject.keywordsLipid Remodelingen_US
dc.subject.keywordsDehydrationen_US
dc.description.acknowledgementThis work was supported by Nanyang Technological University under its Accelerating Creativity and Excellence (ACE) grant (grant no. NTU–ACE2020-07 to W.M., K.J.H and C.H.), Nanyang Technological University start-up grants (grant nos. M4082428 to K.J.H. and M4082352 to C.H.), and Ministry of Education, Singapore, under its Academic Research Fund Tier 1 (grant no. RG92/19).en_US
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:MAE Journal Articles
SBS Journal Articles
SCBE Journal Articles
Files in This Item:
File Description SizeFormat 
Chloroplast_Supplemental Information_PlantPhysio.pdfSupplemental Information807.07 kBAdobe PDFThumbnail
View/Open
Plant_Physiology_manuscript_accepted.pdf333.21 kBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 20

15
Updated on Jul 21, 2024

Web of ScienceTM
Citations 20

6
Updated on Oct 30, 2023

Page view(s)

177
Updated on Jul 20, 2024

Download(s) 50

79
Updated on Jul 20, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.