Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153330
Title: P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC
Authors: Aniweh, Yaw
Gao, Xiaohong
Hao, Piliang
Meng, Wei
Lai, Soak-Kuan
Gunalan, Karthigayan
Chu, Trang T.
Sinha, Ameya
Lescar, Julien
Chandramohanadas, Rajesh
Li, Hoi-Yeung
Sze, Siu Kwan
Preiser, Peter Rainer
Keywords: Science::Biological sciences
Issue Date: 2017
Source: Aniweh, Y., Gao, X., Hao, P., Meng, W., Lai, S., Gunalan, K., Chu, T. T., Sinha, A., Lescar, J., Chandramohanadas, R., Li, H., Sze, S. K. & Preiser, P. R. (2017). P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC. Cellular Microbiology, 19(9), e12747-. https://dx.doi.org/10.1111/cmi.12747
Project: NMRC/1308/2011
RG 152/14
Journal: Cellular Microbiology
Abstract: The successful invasion of Plasmodium is an essential step in their life cycle. The parasite reticulocyte-binding protein homologues (RHs) and erythrocyte-binding like proteins are two families involved in the invasion leading to merozoite-red blood cell (RBC) junction formation. Ca2+ signaling has been shown to play a critical role in the invasion. RHs have been linked to Ca2+ signaling, which triggers the erythrocyte-binding like proteins release ahead of junction formation, consistent with RHs performing an initial sensing function in identifying suitable RBCs. RH5, the only essential RHs, is a highly promising vaccine candidate. RH5-basigin interaction is essential for merozoite invasion and also important in determining host tropism. Here, we show that RH5 has a distinct function from the other RHs. We show that RH5-Basigin interaction on its own triggers a Ca2+ signal in the RBC resulting in changes in RBC cytoskeletal proteins phosphorylation and overall alterations in RBC cytoskeleton architecture. Antibodies targeting RH5 that block the signal prevent invasion before junction formation consistent with the Ca2+ signal in the RBC leading to rearrangement of the cytoskeleton required for invasion. This work provides the first time a functional context for the essential role of RH5 and will now open up new avenues to target merozoite invasion.
URI: https://hdl.handle.net/10356/153330
ISSN: 1462-5814
DOI: 10.1111/cmi.12747
Rights: This is the peer reviewed version of the following article: Aniweh, Y., Gao, X., Hao, P., Meng, W., Lai, S., Gunalan, K., Chu, T. T., Sinha, A., Lescar, J., Chandramohanadas, R., Li, H., Sze, S. K. & Preiser, P. R. (2017). P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC. Cellular Microbiology, 19(9), e12747-, which has been published in final form at https://dx.doi.org/10.1111/cmi.12747. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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