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|Title:||P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC||Authors:||Aniweh, Yaw
Chu, Trang T.
Sze, Siu Kwan
Preiser, Peter Rainer
|Keywords:||Science::Biological sciences||Issue Date:||2017||Source:||Aniweh, Y., Gao, X., Hao, P., Meng, W., Lai, S., Gunalan, K., Chu, T. T., Sinha, A., Lescar, J., Chandramohanadas, R., Li, H., Sze, S. K. & Preiser, P. R. (2017). P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC. Cellular Microbiology, 19(9), e12747-. https://dx.doi.org/10.1111/cmi.12747||Project:||NMRC/1308/2011
|Journal:||Cellular Microbiology||Abstract:||The successful invasion of Plasmodium is an essential step in their life cycle. The parasite reticulocyte-binding protein homologues (RHs) and erythrocyte-binding like proteins are two families involved in the invasion leading to merozoite-red blood cell (RBC) junction formation. Ca2+ signaling has been shown to play a critical role in the invasion. RHs have been linked to Ca2+ signaling, which triggers the erythrocyte-binding like proteins release ahead of junction formation, consistent with RHs performing an initial sensing function in identifying suitable RBCs. RH5, the only essential RHs, is a highly promising vaccine candidate. RH5-basigin interaction is essential for merozoite invasion and also important in determining host tropism. Here, we show that RH5 has a distinct function from the other RHs. We show that RH5-Basigin interaction on its own triggers a Ca2+ signal in the RBC resulting in changes in RBC cytoskeletal proteins phosphorylation and overall alterations in RBC cytoskeleton architecture. Antibodies targeting RH5 that block the signal prevent invasion before junction formation consistent with the Ca2+ signal in the RBC leading to rearrangement of the cytoskeleton required for invasion. This work provides the first time a functional context for the essential role of RH5 and will now open up new avenues to target merozoite invasion.||URI:||https://hdl.handle.net/10356/153330||ISSN:||1462-5814||DOI:||10.1111/cmi.12747||Rights:||This is the peer reviewed version of the following article: Aniweh, Y., Gao, X., Hao, P., Meng, W., Lai, S., Gunalan, K., Chu, T. T., Sinha, A., Lescar, J., Chandramohanadas, R., Li, H., Sze, S. K. & Preiser, P. R. (2017). P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC. Cellular Microbiology, 19(9), e12747-, which has been published in final form at https://dx.doi.org/10.1111/cmi.12747. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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