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https://hdl.handle.net/10356/153474
Title: | Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties | Authors: | Zhao, Zhitong Chua, Huei Min Goh, Bernice Huan Rong Lai, Hui Ying Tan, Shao Jie Moay, Zi Kuang Setyawati, Magdiel Inggrid Ng, Kee Woei |
Keywords: | Engineering::Materials::Biomaterials | Issue Date: | 2022 | Source: | Zhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104. https://dx.doi.org/10.1002/jbm.a.37268 | Project: | H17/01/a0/008 H17/01/a0/0L9 |
Journal: | Journal of Biomedical Materials Research Part A | Abstract: | Human hair keratin (HHK) has been successfully explored as raw materials for three-dimensional scaffolds for soft tissue regeneration due to its excellent biocompatibility and bioactivity. However, none of the reported HHK based scaffolds is able to replicate the strain-stiffening capacity of living tissues when responding to large deformations. In the present study, strain-stiffening property was achieved in scaffolds fabricated from HHK via a synergistic effect of well-defined, aligned microstructure and chemical crosslinking. Directed ice-templating method was used to fabricate HHK-based scaffolds with highly aligned (anisotropic) microstructure while oxidized dopamine (ODA) was used to crosslink covalently to HHKs. The resultant HHK-ODA scaffolds exhibited strain-stiffening behaviour characterized by the increased gradient of the stress-strain curve after the yield point. Both ultimate tensile strength and the elongation at break were enhanced significantly (~700 kPa, ~170 %) in comparison to that of HHK scaffolds lacking of aligned microstructure or ODA crosslinking. In vitro cell culture studies indicated that HHK-ODA scaffolds successfully supported human dermal fibroblasts (HDFs) adhesion, spreading and proliferation. Moreover, anisotropic HHK-ODA scaffolds guided cell growth in alignment with the defined microstructure as shown by the highly organized cytoskeletal networks and nuclei distribution. The findings suggest that HHK-ODA scaffolds, with strain-stiffening properties, biocompatibility and bioactivity, have the potential to be applied as biomimetic matrices for soft tissue regeneration. | URI: | https://hdl.handle.net/10356/153474 | ISSN: | 1549-3296 | DOI: | 10.1002/jbm.a.37268 | Rights: | This is the peer reviewed version of the following article: Zhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104, which has been published in final form at https://doi.org/10.1002/jbm.a.37268. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | Fulltext Permission: | embargo_20230207 | Fulltext Availability: | With Fulltext |
Appears in Collections: | MSE Journal Articles NEWRI Journal Articles |
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JBMR-A-21-0110.R2_Proof_fl.pdf Until 2023-02-07 | 4.22 MB | Adobe PDF | Under embargo until Feb 07, 2023 |
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