Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153474
Title: Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties
Authors: Zhao, Zhitong
Chua, Huei Min
Goh, Bernice Huan Rong
Lai, Hui Ying
Tan, Shao Jie
Moay, Zi Kuang
Setyawati, Magdiel Inggrid
Ng, Kee Woei
Keywords: Engineering::Materials::Biomaterials
Issue Date: 2022
Source: Zhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104. https://dx.doi.org/10.1002/jbm.a.37268
Project: H17/01/a0/008 
H17/01/a0/0L9 
Journal: Journal of Biomedical Materials Research Part A 
Abstract: Human hair keratin (HHK) has been successfully explored as raw materials for three-dimensional scaffolds for soft tissue regeneration due to its excellent biocompatibility and bioactivity. However, none of the reported HHK based scaffolds is able to replicate the strain-stiffening capacity of living tissues when responding to large deformations. In the present study, strain-stiffening property was achieved in scaffolds fabricated from HHK via a synergistic effect of well-defined, aligned microstructure and chemical crosslinking. Directed ice-templating method was used to fabricate HHK-based scaffolds with highly aligned (anisotropic) microstructure while oxidized dopamine (ODA) was used to crosslink covalently to HHKs. The resultant HHK-ODA scaffolds exhibited strain-stiffening behaviour characterized by the increased gradient of the stress-strain curve after the yield point. Both ultimate tensile strength and the elongation at break were enhanced significantly (~700 kPa, ~170 %) in comparison to that of HHK scaffolds lacking of aligned microstructure or ODA crosslinking. In vitro cell culture studies indicated that HHK-ODA scaffolds successfully supported human dermal fibroblasts (HDFs) adhesion, spreading and proliferation. Moreover, anisotropic HHK-ODA scaffolds guided cell growth in alignment with the defined microstructure as shown by the highly organized cytoskeletal networks and nuclei distribution. The findings suggest that HHK-ODA scaffolds, with strain-stiffening properties, biocompatibility and bioactivity, have the potential to be applied as biomimetic matrices for soft tissue regeneration.
URI: https://hdl.handle.net/10356/153474
ISSN: 1549-3296
DOI: 10.1002/jbm.a.37268
Rights: This is the peer reviewed version of the following article: Zhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104, which has been published in final form at https://doi.org/10.1002/jbm.a.37268. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: embargo_20230207
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles
NEWRI Journal Articles

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