Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153474
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dc.contributor.authorZhao, Zhitongen_US
dc.contributor.authorChua, Huei Minen_US
dc.contributor.authorGoh, Bernice Huan Rongen_US
dc.contributor.authorLai, Hui Yingen_US
dc.contributor.authorTan, Shao Jieen_US
dc.contributor.authorMoay, Zi Kuangen_US
dc.contributor.authorSetyawati, Magdiel Inggriden_US
dc.contributor.authorNg, Kee Woeien_US
dc.date.accessioned2021-12-12T06:28:08Z-
dc.date.available2021-12-12T06:28:08Z-
dc.date.issued2022-
dc.identifier.citationZhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104. https://dx.doi.org/10.1002/jbm.a.37268en_US
dc.identifier.issn1549-3296en_US
dc.identifier.urihttps://hdl.handle.net/10356/153474-
dc.description.abstractHuman hair keratin (HHK) has been successfully explored as raw materials for three-dimensional scaffolds for soft tissue regeneration due to its excellent biocompatibility and bioactivity. However, none of the reported HHK based scaffolds is able to replicate the strain-stiffening capacity of living tissues when responding to large deformations. In the present study, strain-stiffening property was achieved in scaffolds fabricated from HHK via a synergistic effect of well-defined, aligned microstructure and chemical crosslinking. Directed ice-templating method was used to fabricate HHK-based scaffolds with highly aligned (anisotropic) microstructure while oxidized dopamine (ODA) was used to crosslink covalently to HHKs. The resultant HHK-ODA scaffolds exhibited strain-stiffening behaviour characterized by the increased gradient of the stress-strain curve after the yield point. Both ultimate tensile strength and the elongation at break were enhanced significantly (~700 kPa, ~170 %) in comparison to that of HHK scaffolds lacking of aligned microstructure or ODA crosslinking. In vitro cell culture studies indicated that HHK-ODA scaffolds successfully supported human dermal fibroblasts (HDFs) adhesion, spreading and proliferation. Moreover, anisotropic HHK-ODA scaffolds guided cell growth in alignment with the defined microstructure as shown by the highly organized cytoskeletal networks and nuclei distribution. The findings suggest that HHK-ODA scaffolds, with strain-stiffening properties, biocompatibility and bioactivity, have the potential to be applied as biomimetic matrices for soft tissue regeneration.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.language.isoenen_US
dc.relationH17/01/a0/008en_US
dc.relationH17/01/a0/0L9en_US
dc.relation.ispartofJournal of Biomedical Materials Research Part Aen_US
dc.rightsThis is the peer reviewed version of the following article: Zhao, Z., Chua, H. M., Goh, B. H. R., Lai, H. Y., Tan, S. J., Moay, Z. K., Setyawati, M. I. & Ng, K. W. (2022). Anisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening properties. Journal of Biomedical Materials Research Part A, 110(1), 92-104, which has been published in final form at https://doi.org/10.1002/jbm.a.37268. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.subjectEngineering::Materials::Biomaterialsen_US
dc.titleAnisotropic hair keratin-dopamine composite scaffolds exhibit strain-stiffening propertiesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.contributor.researchEnvironmental Chemistry and MaterialsCentreen_US
dc.identifier.doi10.1002/jbm.a.37268-
dc.description.versionAccepted versionen_US
dc.identifier.issue1en_US
dc.identifier.volume110en_US
dc.identifier.spage92en_US
dc.identifier.epage104en_US
dc.subject.keywordsHuman Hair Keratinen_US
dc.subject.keywordsDirected Ice Templatingen_US
dc.subject.keywordsOxidized Dopamineen_US
dc.subject.keywordsAnisotropic Microstructureen_US
dc.subject.keywordsBiomimetic Strain-Stiffeningen_US
dc.description.acknowledgementThis research is supported by the Agency for Science, Technology and Research (A*STAR) under its Acne and Sebaceous Gland Program & Wound Care Innovation for the Tropics IAF-PP (H17/01/a0/008 & H17/01/a0/0L9).en_US
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item.grantfulltextembargo_20230207-
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