Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153598
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTay, Joyceen_US
dc.contributor.authorZhao, Yanlien_US
dc.contributor.authorHedrick, James L.en_US
dc.contributor.authorYang, Yi Yanen_US
dc.date.accessioned2021-12-10T08:49:19Z-
dc.date.available2021-12-10T08:49:19Z-
dc.date.issued2021-
dc.identifier.citationTay, J., Zhao, Y., Hedrick, J. L. & Yang, Y. Y. (2021). Elucidating the anticancer activities of guanidinium-functionalized amphiphilic random copolymers by varying the structure and composition in the hydrophobic monomer. Theranostics, 11(18), 8977-8992. https://dx.doi.org/10.7150/thno.60711en_US
dc.identifier.issn1838-7640en_US
dc.identifier.urihttps://hdl.handle.net/10356/153598-
dc.description.abstractRationale: Use of traditional anticancer chemotherapeutics has been hindered by the multifactorial nature of multi-drug resistance (MDR) development and metastasis. Recently, cationic polycarbonates were reported as novel unconventional anticancer agents that mitigated MDR and inhibited metastasis. The aim of this study is to explore structure-anticancer activity relationship. Specifically, a series of cationic guanidinium-based random copolymers of varying hydrophobicity was synthesized with a narrow polydispersity (Ð = 1.12-1.27) via organocatalytic ring-opening polymerization (OROP) of functional cyclic carbonate monomers, and evaluated for anticancer activity, killing kinetics, degradability and functional mechanism. Methods: Linear, branched and aromatic hydrophobic side chain units, such as ethyl, benzyl, butyl, isobutyl and hexyl moieties were explored as comonomer units for modulating anticancer activity. As hydrophobicity/hydrophilicity balance of the polymers determines their anticancer efficacy, the feed ratio between the two monomers was varied to tune their hydrophobicity. Results: Notably, incorporating the hexyl moiety greatly enhanced anticancer efficiency and killing kinetics on cancer cells. Degradation studies showed that the polymers degraded completely within 4-6 days. Flow cytometry and lactate dehydrogenase (LDH) release analyses demonstrated that anticancer mechanism of the copolymers containing a hydrophobic co-monomer was concentration dependent, apoptosis at IC50, and both apoptosis and necrosis at 2 × IC50. In contrast, the homopolymer without a hydrophobic comonomer killed cancer cells predominantly via apoptotic mechanism. Conclusion: The hydrophobicity of the polymers played an important role in anticancer efficacy, killing kinetics and anticancer mechanism. This study provides valuable insights into designing novel anticancer agents utilizing polymers.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.language.isoenen_US
dc.relation.ispartofTheranosticsen_US
dc.rights© 2021 The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.en_US
dc.subjectScience::Chemistryen_US
dc.titleElucidating the anticancer activities of guanidinium-functionalized amphiphilic random copolymers by varying the structure and composition in the hydrophobic monomeren_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doi10.7150/thno.60711-
dc.description.versionPublished versionen_US
dc.identifier.pmid34522222-
dc.identifier.scopus2-s2.0-85114833534-
dc.identifier.issue18en_US
dc.identifier.volume11en_US
dc.identifier.spage8977en_US
dc.identifier.epage8992en_US
dc.subject.keywordsAnticancer Polymersen_US
dc.subject.keywordsGuanidinium-Functionalized Polycarbonates,en_US
dc.description.acknowledgementThis work was funded by the Institute of Bioengineering and Bioimaging (Biomedical Research Council, Agency of Science, Technology and Research, Singapore). J. Tay is grateful to Agency for Science, Technology and Research, Singapore for AGS PhD scholarship.en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SPMS Journal Articles
Files in This Item:
File Description SizeFormat 
v11p8977.pdf1.99 MBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 50

3
Updated on Jun 12, 2024

Web of ScienceTM
Citations 50

2
Updated on Oct 30, 2023

Page view(s)

125
Updated on Jun 14, 2024

Download(s) 50

81
Updated on Jun 14, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.