Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153712
Title: Viable but nonculturable state of yeast candida sp. strain LN1 induced by high phenol concentrations
Authors: Xie, Mengqi
Xu, Luning
Zhang, Rong
Zhou, Yan
Xiao, Yeyuan
Su, Xiaomei
Shen, Chaofeng
Sun, Faqian
Muhammad Zaffar Hashmi
Lin, Hongjun
Chen, Jianrong
Keywords: Engineering::Civil engineering
Issue Date: 2021
Source: Xie, M., Xu, L., Zhang, R., Zhou, Y., Xiao, Y., Su, X., Shen, C., Sun, F., Muhammad Zaffar Hashmi, Lin, H. & Chen, J. (2021). Viable but nonculturable state of yeast candida sp. strain LN1 induced by high phenol concentrations. Applied and Environmental Microbiology, 87(18), e01110-21-. https://dx.doi.org/10.1128/AEM.01110-21
Journal: Applied and Environmental Microbiology
Abstract: Microbial degradation plays an important role in environmental remediation. However, most microorganisms' pollutant-degrading capabilities are weakened due to their entry into a viable but nonculturable (VBNC) state. Although there is some evidence for the VBNC state of pollutant-degrading bacteria, limited studies have been conducted to investigate the VBNC state of pollutant degraders among fungi. In this work, the morphological, physiological, and molecular changes of phenol-degrading yeast strain LN1 exposed to high phenol concentrations were investigated. The results confirmed that Candida sp. strain LN1, which possessed a highly efficient capability of degrading 1,000 mg/liter of phenol as well as a high potential for aromatic compound degradation, entered into the VBNC state after 14 h of incubation with 6,000 mg/liter phenol. Resuscitation of VBNC cells can restore their phenol degradation performance. Compared to normal cells, significant dwarfing, surface damage, and physiological changes of VBNC cells were observed. Molecular analysis indicated that downregulated genes were related to the oxidative stress response, xenobiotic degradation, and carbohydrate and energy metabolism, whereas upregulated genes were related to RNA polymerase, amino acid metabolism, and DNA replication and repair. This report revealed that a pollutant-degrading yeast strain entered into the VBNC state under high concentrations of contaminants, providing new insights into its survival status and bioremediation potential under stress. IMPORTANCE The viable but nonculturable (VBNC) state is known to affect the culturability and activity of microorganisms. However, limited studies have been conducted to investigate the VBNC state of other pollutant degraders, such as fungi. In this study, the VBNC state of a phenol-degrading yeast strain was discovered. In addition, comprehensive analyses of the morphological, physiological, and molecular changes of VBNC cells were performed. This study provides new insight into the VBNC state of pollutant degraders and how they restored the activities that were inhibited under stressful conditions. Enhanced bioremediation performance of indigenous microorganisms could be expected by preventing and controlling the formation of the VBNC state.
URI: https://hdl.handle.net/10356/153712
ISSN: 0099-2240
DOI: 10.1128/AEM.01110-21
Rights: © 2021 American Society for Microbiology. All Rights Reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:CEE Journal Articles
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